《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (2): 125-128.

• 论文 • 上一篇    下一篇

α-玉米赤霉醇改善Aβ诱导痴呆模型小鼠认知行为的实验研究

单玉芳，任志丽，左萍萍   

  1. 1.吉林省白城市医院妇产科，吉林白城市137000；2.中国医学科学院基础医学研究所，北京市100005。
  • 收稿日期:2013-07-26 修回日期:1900-01-01 出版日期:2014-02-25 发布日期:2014-02-25
  • 通讯作者: 左萍萍

Effects of α-Zearalanol on Cognitive Impairment Induced by β-amyloid in Mice

SHAN Yu-fang, REN Zhi-li, ZUO Ping-ping.   

  1. Department of Gynecology and Obstetrics, Baicheng Hospital, Baicheng 137000, Jilin, China
  • Received:2013-07-26 Revised:1900-01-01 Published:2014-02-25 Online:2014-02-25

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摘要： 目的观察α-玉米赤霉醇(α-ZAL)对阿尔茨海默病(AD)小鼠模型认知行为的改善作用及其机制。方法将40只成年雌 性小鼠随机分为对照组、模型组、苯甲酸雌二醇(EB)组和α-ZAL组，每组10 只。除对照组外，其他组于实验开始后第7 天于脑室 内注射β-淀粉样蛋白(Aβ25-35)3 nmol 造成拟AD痴呆模型。对照组和模型组肌肉注射橄榄油0.05 ml/d，共14 d；EB组肌肉注射EB0.1 nmol/d，共14 d；α-ZAL组肌肉注射α-ZAL 0.1nmol/d，共14 d。Morris 水迷宫检测小鼠空间学习记忆能力。分光光度法和同位素法测脑内氧化应激指标和一氧化氮(NO)系统改变。结果模型组Morris 水迷宫末次寻台潜伏期明显延长(P<0.01)，EB 组和α-ZAL组较模型组缩短(P<0.05)。模型组脑内过氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)下降(P<0.05)，丙二醛(MDA)、原生型一氧化氮合酶(cNOS)、诱生型一氧化氮合酶(iNOS)和NO上升(P<0.05)；除α-ZAL组海马SOD和cNOS外(P>0.05)，EB组和α-ZAL组各指标均优于模型组(P<0.01)。EB组和α-ZAL组之间无显著性差异(P>0.05)。结论α-ZAL和EB一样能通过提高脑内抗氧化和降低脂质过氧化能力，拮抗Aβ和NO的神经毒性，明显改善AD模型小鼠的认知行为。

关键词: 阿尔茨海默病, α-玉米赤霉醇, Morris 水迷宫, 抗氧化, 一氧化氮, 小鼠

Abstract: Objective To explore the effect of α-zearalanol (α-ZAL) on β-amyloid (Aβ) induced mice and the mechanism. Methods The model was induced by intracerebroventricular injection of Aβ25-35. The mice were divided randomly into sham group, model group, estradiol benzoate (EB) group (Aβ+EB) as a positive control and α-ZAL (Aβ+α-ZAL) group. Morris water maze was used to evaluate the learning and memory ability. The levels of antioxidant enzymes and nitric oxide system in the brain tissue were detected with spectrophotometric and sotopic method. Results The escape latency was longer in the model group than in the control group (P<0.01), and was shorter in the EB group and α-ZAL group than in the model group (P<0.05). Compared with the control group, the level of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) decreased, and the level of malonaldehyde (MDA), constitutive nitric oxide synthase (cNOS), inducible nitrous oxide synthesis (iNOS), and nitric oxide (NO) increased in the model group (P<0.05); compared with the model group, the level of
SOD and GSH-Px increased, and the level of MDA, cNOS, iNOS and NO decreased in the EB group and α-ZAL group (P<0.05), except the level of SOD and cNOS in hippocampus in α-ZAL group (P>0.05). There was no significant difference between EB group and α-ZAL group (P>0.05). Conclusion α-ZAL could improve the cognitive behavior in Aβ25- 35 induced mice by increasing the antioxidant activities and decreasing the lipid peroxidation.

Key words: Alzheimer's disease, α-Zearalanol, Morris water maze, antioxidant, nitric oxide, mice