《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (3): 215-220.

• 论文 • 上一篇    下一篇

电针对兔腰肌急性钝挫伤后组织修复与碱性成纤维细胞生长因子/细胞外信号调节激酶信号通路的影响

陈欢，彭博，李富运，于雪，卢虎英，卫肖艳，彭园园，范伊凡，张莉   

  1. 1.北京中医药大学针灸推拿学院，北京市100029；2.北京中医药大学基础医学院科研实验中心，北京市100029；3.中国康复研究中心，北京市100068。
  • 收稿日期:2013-08-09 修回日期:2013-10-08 出版日期:2014-03-25 发布日期:2014-03-25
  • 通讯作者: 张莉

Effects of Electroacupuncture on Morphology Repairment and Basic Fibroblast Growth Factor/Extracellular Signal-regulated Kinase Signal Pathway of Rabbits with Acute Lumbar Muscle Contusion

CHEN Huan, PENG Bo, LI Fu-yun, et al.   

  1. Acupuncture Moxibusion and Tuina School, Beijing University of Chinese Medcine, Beijing 100029, China
  • Received:2013-08-09 Revised:2013-10-08 Published:2014-03-25 Online:2014-03-25

PDF (PC)

被引次数

17

摘要：

目的观察电针对兔腰肌急性钝挫伤后碱性成纤维细胞生长因子(bFGF)、结蛋白(Desmin)以及细胞外信号调节激酶(ERK1/2)表达的影响，探讨电针对兔腰肌损伤后组织再生修复影响的可能机制。方法40 只雄性新西兰大耳兔随机分为空白组、模型组、电针委中组、电针局部组，每组10 只。采用钝挫伤方法造成兔腰肌损伤模型并对各组动物进行外表观察评分。造模后电针治疗2 周，HE染色观察腰肌组织形态改变，免疫组化染色观察腰肌bFGF的表达，Western blotting 法检测腰肌Desmin 与ERK1/2蛋白表达。结果模型组、电针委中组与电针局部组外表观察评分均明显高于空白组(P<0.01)。组织形态学评分由高到低依次为模型组、电针局部组、电针委中组、空白组(P<0.05)，腰肌bFGF的表达依次为电针局部组、电针委中组、模型组、空白组，Desmin的表达为电针局部组=电针委中组、空白组=模型组(P<0.05)，ERK1/2 的表达为电针局部组=电针委中组、模型组、空白组(P<0.05)。结论电针可能通过上调bFGF/ERK信号通路的表达，促进兔腰肌急性钝挫伤后的组织修复。

关键词: 腰肌急性钝挫伤, 电针, 委中, 碱性成纤维细胞生长因子, 结蛋白, 细胞外信号调节激酶, 兔

Abstract: Objective To observe the effect of electroacupuncture on basic fibroblast growth factor (bFGF), Desmin and extracellular signal-regulated kinase (ERK1/2) expression of rabbits with acute lumbar muscle contusion, and discuss the possible mechanism of muscle tissue regeneration and repair. Methods 40 male New Zealand rabbits were randomly divided into blank group (BG, n=10), model group (MG, n=10), Weizhong (BL40) electroacupuncture group (WG, n=10) and local electroacupuncture group (LG, n=10). Lumbar muscle injury was established with blunt trauma, and the rabbits were assessed with Appearance Score. The WG and LG accepted electroacupuncture for 2 weeks after modeling. The lumbar muscle of each animal was stained with HE to observe pathological changes, and immunohistochemical staining was used to observe bFGF expression, and Western blotting was used to detect the expression of Desmin and ERK1/2 protein.Results Appearance Score was more in the MG, WG and LG than in the BG (P<0.01). Histological score ranked from more to less as MG,LG, WG and BG (P<0.05). The expression of bFGF ranked as LG, WG, MG and BG (P<0.01), Desmin ranked as WG=LG and BG=MG (P<0.05), ERK1/2 as LG=WG, MG and BG (P<0.05). Conclusion Electroacupuncture could promote the regeneration and repair of tissue after acute lumbar muscle contusion, which may be related to the up-regulation of bFGF/ERK signaling pathway.

Key words: lumbar acute contusion, electroacupuncture, BL40, basic fibroblast growth factor, Desmin, extracellular signal-regulated protein kinase, rabbits