《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (3): 236-239.

• 论文 • 上一篇    下一篇

葡萄籽原花青素对睡眠呼吸暂停低氧大鼠海马区超微结构及认知功能的影响

赵雅宁，刘文倩，曹书华，郭霞，王红阳   

  1. 1.天津中医药大学，天津市300000；2.河北联合大学康复医学院，河北唐山市063000；3.天津市第一中心医院，天津市300000。
  • 收稿日期:2013-05-23 修回日期:2013-07-23 出版日期:2014-03-25 发布日期:2014-03-25
  • 通讯作者: 曹书华

Effect of Grape Seed Proanthocyanidin Extract on Ultrastructure Injury in Hippocampous and Cognition Impairment in Rats of Obstructive Sleep Apnea Hypoxia

ZHAO Ya-ning, LIU Wen-qian, CAO Shu-hua, et al.   

  1. Tianjin University of Traditional Chinese Medicine,Tianjin 300000, China
  • Received:2013-05-23 Revised:2013-07-23 Published:2014-03-25 Online:2014-03-25

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被引次数

23

摘要： 目的探讨葡萄籽原花青素(GSPE)对睡眠呼吸暂停低氧大鼠海马区超微结构及认知功能的影响。方法80 只雄性Sprague-Dawley 大鼠随机分成对照组，模型组，GSPE高、低剂量组。对照组暴露于空气中，模型组每天暴露于低氧条件下(50 ml/L) 8 h，持续时间2 周和6 周，GSPE高、低剂量组入舱前2 周开始每天分别灌胃给药GSPE 200 mg/kg、100 mg/kg。电镜观察海马区神经细胞超微结构，比色法检测大脑组织丙二醛(MDA)水平和超氧化物歧化酶(SOD)水平，TUNEL法检测凋亡细胞，水迷宫测试动物学习记忆功能。结果模型组海马区超微结构损伤，MDA含量显著升高、SOD活性显著降低、TUNEL阳性细胞显著增多，水迷宫检测动物逃避潜伏期时间显著延长、穿台次数显著减少(P<0.001)；与模型组比较，GSPE各组海马区损伤减轻， MDA含量降低，SOD活性提高，TUNEL阳性细胞减少，水迷宫测试逃避潜伏期时间缩短、穿台次数增多(P<0.05)；高剂量组优于低剂量组(P<0.05)。结论葡萄籽原花青素减轻睡眠呼吸暂停模式低氧大鼠海马区超微结构的损伤，改善认知功能。

关键词: 睡眠呼吸暂停, 学习, 记忆, 海马, 丙二醛, 超氧化物歧化酶, 凋亡, 大鼠

Abstract: Objective To investigate the effect of grape seed proanthocyanidin extract (GSPE) on ultrastructure injury in hippocampous and cognition impairment in rat model of obstructive sleep apnea hypoxia. Methods 80 male Sprague-Dawley rats were randomly divided into control group, model group, high and low dose GSPE groups. The control group was exposed in air, while the model group was suffered from intermittent hypoxia conditions (50 ml/L, 8 h everyday, for 2 or 6 weeks), and the GSPE groups accepted GSPE 200 mg/kg or 100 mg/kg 2 weeks respectively before hypoxia. Pathology in hippocampal region was observed under electromicroscope. Malondialdehyde (MDA) contents and superoxide dismutase (SOD) activity were detected with colorimetry, and apoptotic cells were measured with TUNEL. The cognition function of rats was assessed with the Morris water maze (MWM). Results The ultrastructure in hippocampous was significantly injured,with the increase of MDA and decrease of SOD (P<0.001) in the model group. The apoptotic cells increased (P<0.001). The escaping latency prolonged (P<0.001) and the frequency of crossing the platform decreased (P<0.001) in MWM test in the model group. Compared with the model group, the GSPE groups decreased in MDA content, increased in SOD level, decreased in apoptotic cells and ultrastructure damages, shortened the escaping latency, and increased the frequency of crossing the platform (P<0.001), especially in the high dose group (P<0.05). Conclusion GSPE can relieve the damage of ultrastructure and improve cognition function after obstructive sleep apnea hypoxia in rats.

Key words: sleep apnea, learning, memory, hippocampus, malondialdehyde, superoxide dismutase, apoptosis, rats