《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (5): 434-441.

• 论文 • 上一篇    下一篇

单核细胞趋化蛋白-1对大鼠脑内神经干细胞的诱导作用

张浩，丁鹏，王进昆，苏稳，牟临杰，王波，龚光辉，刘景传，王崇谦   

  1. 昆明医科大学附属第一医院神经外科，云南昆明市650032。
  • 收稿日期:2013-08-27 修回日期:2013-11-05 出版日期:2014-05-25 发布日期:2014-05-25
  • 通讯作者: 王崇谦

Effect of Exogenous Monocyte Chemoattractant Protein-1 on Neural Stem Cells in Brain of Rats

ZHANG Hao, DING Peng, WANG Jin-kun, et al.   

  1. Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China
  • Received:2013-08-27 Revised:2013-11-05 Published:2014-05-25 Online:2014-05-25

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被引次数

1

摘要： 目的探讨外源性单核细胞趋化蛋白-1(MCP-1)对体内神经干细胞的作用。方法成年雄性Sprague-Dawley 大鼠64 只分为空白组(n=4)、对照组(n=30)和实验组(n=30)。空白组不做处理，对照组大脑皮层微量定向注射PBS 液，实验组注射MCP-1。注射后3 d、5 d、7 d、14 d、21 d 分别取对照组和实验组大鼠各6 只灌注取脑。免疫组织化学法观察脑内不同部位nestin 阳性细胞的分布。结果实验组皮质区、海马区nestin 阳性细胞矫正累积吸光度随时间延长而显著增加(P<0.001)。对照组和空白组之间无显著性差异(P>0.05)。结论外源性MCP-1 可以促进MCP-1 富集部位的nestin阳性细胞的数量增多。

关键词: 单核细胞趋化因子-1, 神经干细胞, 趋化因子, nestin

Abstract: Objective To apply the exogenous monocyte chemoattractant protein-1 (MCP-1) to induce the neural stem cells in vivo.Methods 64 adult male Sprague-Dawley rats were randomly divided into blank (n=4), control (n=30) and experimental (n=30) groups. The
experimental group was injected with MCP-1 into the cerebra, and the control group with PBS, and the blank group with no intervention.The number of nestin positive cells in brain was observed with immunohistochemistry 3, 5, 7, 14, 21 days after injection. Results The number of nestin positive cells increased with time in the cortex and hippocampus in the experimental group (P<0.001). There was no significant difference between the control group and the blank group (P>0.05). Conclusion Exogenous MCP-1 may induce the increase of neural stem cells in vivo.

Key words: monocyte chemoattractant protein-1, neural stem cells, chemotactic factor, nestin