《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (6): 527-532.

• 论文 • 上一篇    下一篇

改良激素法建立早期股骨头坏死动物模型

闵红巍，刘克敏，王安庆，韩新祚，顾蕊
   

  1. 1. 首都医科大学康复医学院，北京市100068；2. 中国康复研究中心北京博爱医院骨科，北京市100068。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-06-25 发布日期:2014-06-25

EstablishmentofAnimalModelofEarlyOsteonecrosisoftheFemoralHeadwithModified-inducedSteroidMethod

MINHong-wei, LIUKe-min,WANGAn-qing,etal.   

  1. CapitalMedicalUniversitySchoolofRehabilitationMedicine,DepartmentofOrthopedics,BeijingBo'ai Hospital,ChinaRehabilitationResearchCenter,Beijing100068,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-06-25 Online:2014-06-25

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摘要： 目的研究早期激素性股骨头坏死(ONFH)动物模型的建立方法。方法健康雄性比格犬20只，随机分为对照组(n=10)和实验组(n=10)。实验组肌注大肠杆菌内毒素10μg/kg，然后连续3d肌注甲基泼尼松龙20mg/kg；对照组肌注等量生理盐水。两组分别于给药后2个月、4个月进行MRI检查，然后每组各处死5只动物，切取双侧股骨头标本做组织学检查。两组均在注射药物前后24h抽静脉血化验血浆凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、抗凝血酶-Ⅲ(AT-Ⅲ)。结果实验组给药后2个月病理结果显示，有4髋发生ONFH，MRI未显示异常；给药后4个月病理结果显示，有6髋发生ONFH，MRI未显示异常。实验组注射药物后PT、APTT、AT-Ⅲ值显著缩短(P<0.001)，且显著短于对照组(P<0.001)。结论改良激素法可以建立早期股骨头坏死的动物模型，高凝低纤溶可能是激素性骨坏死的原因。

关键词: 股骨头坏死, 早期, 改良激素法, 动物模型, 比格犬

Abstract: ObjectiveToexplorethemethodtoestablishanimalmodelofearlyosteonecrosisofthefemoralhead(ONFH)inducedby steroid.Methods20healthymaleBeagleswererandomlydividedintocontrolgroupandexperimentalgroupwith10dogsineachgroup. Theexperimentalgroupwasinjectedlipopolysaccharide10μg/kgandmethylprednisolone20mg/kgfor3daysconsecutively.Thecontrol groupwasinjectednormalsaline.2monthsand4monthsafteradministration,bothgroupswereperformedmagneticresonanceimaging (MRI).5animalsweresacrificedrespectivelyat2monthsand4monthsafteradministrationineachgroup,andbilateralfemoralheadspeci-menswereobtainedtoperformhistologicalexamination.Plasmaprothrombintime(PT),activatedpartialthromboplastintime(APTT),anti-thrombinIII(AT-III)weretestedbeforeand24hafteradministration.ResultsIntheexperimentalgroup,thepathologicalresultsshowed thattherewere4ONFH2monthsand6ONFH4monthsafteradministrationandMRIdidnotshowanyabnormality.Comparedwiththe controlgroup,thePT,APTT,AT-IIIintheexperimentgroupshortenedsignificantlyafteradministration(P<0.001).ConclusionModified steroid-inducedmethodcanestablishtheanimalmodelofearlyONFH.Hypercoagulationandlowfibrinolysismaybethereasonofste-roid-inducedosteonecrosis.

Key words: osteonecrosisofthefemoralhead, earlystage, modifiedsteroid-inducedmethod, animalmodel, Beagles