《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (7): 621-625.

• 论文 • 上一篇    下一篇

侧脑室内连续注射神经肽S对神经病理性疼痛大鼠疼痛及焦虑行为的影响

金旭，曾敏，熊蔚，罗芳   

  1. 首都医科大学附属北京天坛医院麻醉科，北京市100050。
  • 收稿日期:2014-03-14 修回日期:2014-04-11 出版日期:2014-07-25 发布日期:2014-07-25

Effect of Intracerebroventricular Injection of Neuropeptide S on Pain and Anxiety Behaviors in Rats with Neuropathic Pain

JIN Xu, ZENG Min, XIONG Wei, et al.   

  1. Department of Anesthesia and Pain Therapy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
  • Received:2014-03-14 Revised:2014-04-11 Published:2014-07-25 Online:2014-07-25

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摘要： 目的研究侧脑室内连续注射神经肽S对神经病理性疼痛大鼠疼痛行为及焦虑行为的影响，探讨神经肽S对疼痛和焦虑影响的作用机制。方法40 只雄性Sprague-Dawley 大鼠被随机分为假手术对照组(Sham-Veh 组)、假手术神经肽S 组(Sham-NPS组)、慢性压迫性神经损伤组(CCI 组)、神经肽S 低剂量组(NPSl 组，0.1 nmol/L)和神经肽S 高剂量组(NPSh 组，1 nmol/L)，每组8只。制备CCI动物模型，造模成功后第7 天左侧侧脑室置入微透析用导针，自术后第8 天起每日侧脑室注射相应药物，在术后第14天检测机械痛缩足阈值和热辐射缩足潜伏期，并通过高架十字迷宫检测焦虑行为的变化。采用免疫组织荧光技术检测左侧杏仁核区神经肽S受体(NPSR)的表达。结果与Sham-Veh 组比较，CCI组大鼠术后14 d 出现明显疼痛和焦虑行为(P<0.01)，NPSh组明显缓解(P<0.01)，NPSl组与CCI组比较无显著性差异(P>0.05)。免疫组织荧光结果显示，与CCI组相比，NPSh组杏仁核区NPSR表达明显增加。结论神经肽S可以剂量依赖性地逆转CCI大鼠的疼痛和焦虑行为，其机制可能与杏仁核NPSR上调有关。

关键词: 神经病理性疼痛, 神经肽S, 焦虑, 侧脑室注射, 杏仁核, 大鼠

Abstract: Objective To investigate the effects of intracerebroventricular injection of neuropeptide S (NPS) on pain and anxiety behaviors in rats with neuropathic pain, and explore the possible mechanism. Methods 40 male Sprague-Dawley rats were randomly divided into Sham-Vehicle group, Sham-NPS group, chronic constriction injury (CCI) group, Neuropeptide S low-dose group (NPSl group, 0.1 nmol/L) and Neuropeptide S high-dose group (NPSh group, 1 nmol/L), with 8 rats in each group. Pain-related behaviors, anxiety-related behaviours,and expression of NPS receptor (NPSR) in the left amygdaloid nucleus were measured on the 14th day via intracerebroventricular injection.Results Compared with the Sham-Vehicle group, the CCI group demonstrated significant pain and anxiety behaviors 14 days after operation (P<0.01), the NPSh group significantly relieved (P<0.01). And there was no significant difference between the NPSl group and the CCI group (P>0.05). Compared with the CCI group, NPSR expression in amygdaloid nucleus increased in the NPSh group. Conclusion NPS can dose-dependently relieve the pain and anxiety behaviors in CCI rats, which may be related with the increase of NPSR in amygdaloid nucleus.

Key words: neuropathic pain, neuropeptide S, anxiety, intracerebroventricular injection, amygdaloid nucleus, rats