《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (8): 713-717.

• 专题 • 上一篇    下一篇

原花青素对急性脊髓损伤大鼠核因子-кB和白细胞介素-6 表达的影响

马延仃，谢少华，杨拯，杨海燕，李晓，张艺，梁羽，郭秀娟，张奇兰，张晓
   

  1. 成都医学院，四川成都市610500
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-08-25 发布日期:2014-08-25

Effect of Proanthocyanidin on Expression of Nuclear Factor kappa B and Interleukin-6 in Rats after Spinal Cord Injury

MA Yanding,XIE Shao-hua, YANG Zheng, YANG Hai-yan, LI Xiao, ZHANG Yi, LIANG Yu, GUO Xiu-juan, ZHANG Qi-lan, ZHANG Xiao.
  

  1. ChengduMedical College, Chengdu, Sichuan 610500, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-08-25 Online:2014-08-25

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摘要： 目的观察原花青素对脊髓损伤后大鼠核因子-кB (NF-кB)、白细胞介素-6 (IL-6)表达变化的影响。方法36 只健康成年Sprague-Dawley 大鼠均分为3 组：原花青素治疗组(A组)、甲基泼尼松龙治疗组(B 组)和对照组(C 组)。采用Allen 法复制大鼠T9急性脊髓损伤模型，于术后1 d、3 d 和7 d 对各组大鼠行后肢运动功能BBB评分和斜板试验；免疫组织化学染色检测脊髓NF-кB和IL-6 的表达。结果术后3 d、7 d，A组、B组大鼠BBB评分和斜板试验成绩均优于C组(P<0.05)。A组术后各时间点NF-кB表达均明显低于C组(P<0.01)，B组术后3 d、7 d 的NF-кB表达强度明显低于C组(P<0.01)。术后各时间点，A、B两组IL-6 表达均低于C组(P<0.05)。结论原花青素可抑制脊髓损伤后大鼠脊髓组织NF-кB、IL-6 的表达，有效抑制炎症反应，从而促进大鼠后肢运动功能的恢复。

关键词: 脊髓损伤, 原花青素, 运动功能, 核因子-кB, 白细胞介素-6, 大鼠

Abstract: Objective To observe the effect of proanthocyanidin on the expression of nuclear factor kappa B (NF-кB) and interleukin-6 (IL-6) in rats with spinal cord injury (SCI). Methods 36 healthy adult Sprague-Dawley rats were randomly divided into proanthocyanidin treatment group (group A, n=12), methylprednisolone (MP) treatment group (group B, n=12) and model control group (group C, n=12). Allen's method was used to establish the model of acute spinal cord injury in T9. On the 1st, 3rd and 7th days after operation, 4 rats in each group were assessed with Basso-Beattie-Bresnahan (BBB) scale and Slanting Board Test (SBT), and the expression of NF-кB and IL-6 of the spinal cord were detected with immunohistochemistry. Results The scores of BBB and SBT were better in groups A and B than in the group C 3 and 7 days after SCI (P<0.05). Compared with group C, the expression of NF-кB in group A decreased all the time after SCI (P<0.01), and 3 and 7 days after SCI in group B (P<0.01); while the expression of IL-6 decreased all the time after SCI in groups A and B (P<0.05). Conclusion Proanthocyanidin may inhibit the expression of NF-кB and IL-6 in spinal cord after SCI in order to reduce inflammation and improve the hindlimb motor function in rats after SCI.

Key words: spinal cord injury, proanthocyanidin, motor function, nuclear factor kappa B, interleukin-6, rats