《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (8): 718-722.

• 专题 • 上一篇    下一篇

大鼠脊髓损伤后P物质与神经源性肠道功能障碍的关系

龙志华，高飞，张锋良，岳军忠，王磊，王烨，刘文国，徐青
   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心北京博爱医院，北京市100068
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-08-25 发布日期:2014-08-25

Substance P in Neurogenic Bowel Dysfunction after Spinal Cord Injury in Rats

LONG Zhi-hua, GAO Fei, ZHANG Feng-liang, YUE Jun-zhong, WANG Lei, WANG Ye, LIU Wen-guo, XU Qing.
  

  1. Capital Medical University School of Rehabilitation Medicine, Department of General Surgery, Beijing Bo'ai Hospital, China Rehabilitation Reserch Center, Beijing 100068, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-08-25 Online:2014-08-25

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摘要： 目的探讨脊髓损伤后结肠中P 物质与神经源性肠道功能障碍的关系。方法60 只体质量(220±40) g 的雄性Sprague-Dawley 大鼠随机分为假手术组(n=20)、正常对照组(n=20)和模型组(n=20)。氯胺酮60 mg/kg 腹腔注射麻醉大鼠，利用NYU脊髓打击器，以75 g &#8901;cm致伤力制作T10脊髓损伤模型，分别于造模后24 h、1 周、3 周和5 周时切除大鼠结肠组织制作标本，检测肠道传输功能，采用ELISA 方法测定血清中和组织中的P 物质含量，实时荧光定量PCR和Western blotting 法检测P 物质mRNA和蛋白表达。结果模型组大鼠脊髓损伤后出现肠道传输功能下降，且于造模后3 周时肠道传输达到最低值；造模后3 周时模型组血清和组织中P 物质含量与假手术组相比均降低，结肠组织中P 物质的mRNA及蛋白表达水平也下调，与假手术组、正常对照组相比具有显著性差异，假手术组P 物质的表达是模型组的(3.12±0.51)倍(P<0.05)。结论大鼠脊髓损伤后神经源性肠道功能障碍与结肠中P物质的表达降低有关。

关键词: 脊髓损伤, P物质, 神经源性肠道功能障碍, 大鼠

Abstract: Objective To investigate the relationship between neurogenic bowel dysfunction (NBD) and substance P in rats suffering from spinal cord injury (SCI). Methods 60 male Sprague-Dawley rats, weighted (220±40) g, were randomly divided into three groups: sham group (n=20), normal group (n=20) and model group (n=20) and then were subdivided into subgroups of 24 h, 1 week, 3 weeks, and 5 weeks respectively after SCI. SCI model was established at thoracic 10 segment of rat with NYU impactor device. The colon tissue of the rats was resected and stored. Substance P in serum and tissue was measured by ELISA. The tissue was examined by real-time RT-PCR and Western blotting to analyze the expression of substance P. Results The colon intestinal transmission function decreased and delineated at minimum value at 3 weeks in the model group. There was statistical significance with respect to the content of substance P in serum and tissue between the sham group and model group at 3 weeks. The expression of substance P in the sham group was (3.12±0.51) times of the model group (P<0.05). Conclusion Substance P may take part in NBD after SCI in rats.

Key words: spinal cord injury, substance P, neurogenic bowel dysfunction, rats