《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (9): 823-826.

• 基础研究 • 上一篇    下一篇

即时高压氧治疗对大鼠脑缺血后脑水肿及水通道蛋白4 表达的影响

陈方圆，于敏，张立新，张志强
   

  1. 中国医科大学附属盛京医院康复科，辽宁沈阳市110022
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-25 发布日期:2014-09-25

Effects of Instant Hyperbaric Oxygen Therapy on Brain Edema and Expression of Aquaporin-4 after Cerebral Ischemia in Rats

CHEN Fang- yuan, YU Min, ZHANG Li- xin, ZHANG Zhi- qiang.
  

  1. Rehabilitation Center, Shengjing Hospital, China Medical University,Shenyang, Liaoning 110022, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-25 Online:2014-09-25

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摘要： 目的观察即时高压氧治疗对缺血早期脑水肿形成的影响，并探讨其可能的机制。方法健康成年雄性清洁级Sprague-Dawley 大鼠18 只，随机分为假手术组(n=6)、对照组(n=6)和高压氧组(n=6)。对照组及高压氧组采用线栓法制备大鼠大脑中动脉阻塞(MCAO)模型，其中高压氧组在舱内升压时进行模型制备并予高压氧治疗60 min。各组均于术后6 h 行Longa 评分法评分，采用干湿比重法检测脑组织含水量，免疫组化SP 法观察水通道蛋白4 (AQP4)表达，应用图像分析系统进行半定量分析。结
果与对照组比较，高压氧组大鼠神经功能评分改善(P<0.05)，缺血半球脑含水量减少(P<0.05)，AQP4 表达量减少(P<0.05)。结论即时高压氧治疗可有效缓解脑缺血后症状，对脑水肿的发生有抑制作用；AQP4 表达减少可能是其机制之一。

关键词: 脑缺血, 高压氧, 神经功能评分, 脑水肿, 水通道蛋白4, 大鼠

Abstract: Objective To observe the effects of instant hyperbaric oxygen (HBO) therapy on brain edema and aquaporin-4 (AQP4) expression after cerebral ischemia in rats. Methods 18 healthy adult male Sprague-Dawley rats were randomly divided into sham group (n=6),control group (n=6) and HBO group (n=6). Middle cerebral artery occlusion model was established with modified Longa method in the control group and HBO group. HBO was administered after cerebral ischemia immediately in HBO group for 60 min. They were observed with the neurological function score, brain tissue water content and AQP4 experssion 6 h after operation. Results Compared with the control group, the neurological score in HBO group improved significantly (P<0.05), while the brain tissue water content and brain AQP4 expression decreased (P<0.05). Conclusion HBO may improve the neurological function and brain edema after cerebral ischemia, which may relate with inhibiting the expression of AQP4.

Key words: cerebral ischemia, hyperbaric oxygen, neurological score, brain edema, aquaporin-4, rats