《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (9): 831-834.

• 基础研究 • 上一篇    下一篇

电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响

林浴坤1，陶静1，陈斌1，林如辉2
   

  1. 1.福建中医药大学康复医学院，福建福州市350108；2.福建中西医结合研究院，福建福州市350108。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-25 发布日期:2014-09-25

Effect of Electroacupuncture on Learning and Memory Ability of Cerebral Ischemia/Reperfusion Injured Rats

LIN Yu-kun, TAO Jing, CHEN Bin, LIN Ru- hui.
  

  1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-25 Online:2014-09-25

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摘要： 目的探讨电针神庭、百会对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响及其机制。方法雄性Sprague-Dawley大鼠45 只，随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组、电针组用线栓法制备局灶性脑缺血2 h 再灌注损伤模型。电针组行电针干预7 d。各组在造模后3 d 开始行Morris 水迷宫测试。7 d 后尼氏染色观察海马神经细胞变化，逆转录PCR检测Bcl-2、Bax mRNA表达。结果电针组逃避潜伏期显著低于模型组(P<0.001)，穿过平台次数显著低于模型组(P=0.001)。电针组海马区细胞损伤及Bax mRNA水平降低、Bcl-2 mRNA水平提高(P<0.05)。结论电针能改善局灶性脑缺血再灌注损伤大鼠学习记忆能力，保护神经细胞，其机制可能与调节凋亡相关基因Bax、Bcl-2 的表达有关。

关键词: 脑缺血/再灌注, 电针, 学习记忆, 凋亡, Bax, Bcl-2

Abstract: Objective To investigate the effect of electroacupuncture at Shenting (DU24) and Baihui (DU20) on learning and memory ability, as well as the nerve cell and the expression of mRNA of Bcl-2 and Bax in the hippocampus of rats with cerebral ischemia/reperfusion (I/R) injury. Methods 45 male Sprague-Dawley rats were randomly diveded into sham group (n=15), control group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 2 h and reperfusion. The electroacupuncture group received electroacupuncture at Shenting and Baihui for 7 days. They were tested with Morris water maze, and then, their hippocampus was observed under Nissl staining and the level of Bcl-2 and Bax mRNA were determined with RT-PCR. Results The escape latency decreased (P<0.001) and the frequence the platform was crossed increased (P=0.001) in the electroacupuncture group compared with the model group, with less damage of the nerve cell and the expression of Bax mRNA, and more of Bcl-2 mRNA (P<0.05). Conclusion Electroacupuncture can prevent learning and memory ability from cerebral ischemia/reperfusion injury in rats, which may associate with adjustment of expression of apoptosis-related gene to inhibit apoptosis of nerve cell.

Key words: cerebral ischemia/reperfusion, electroacupuncture, learning and memory, apoptosis, Bax, Bcl-2