《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (9): 839-841.

• 基础研究 • 上一篇    下一篇

胰岛素面部注射对大鼠面神经挤压伤的效果

张玉花1，王华明2，尹洁3，景玉宏3
   

  1. 1.甘肃广播电视大学，甘肃兰州市730030；2.甘肃省中医院儿骨科，甘肃兰州市730050；3.兰州大学基础医学院人体解剖与组织胚胎学研究所暨神经科学研究所，甘肃兰州市730000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-25 发布日期:2014-09-25

Effect of Local Insulin Injection on Crushed Injury of Facial Nerve in Rats

ZHANG Yu- hua, WANG Hua- ming, YIN Jie, JING Yu-hong.
  

  1. Gansu Radio & TV University, Lanzhou, Gansu 730030, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-25 Online:2014-09-25

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被引次数

1

摘要： 目的探讨胰岛素面部注射对大鼠面神经挤压伤神经纤维的再生及神经损伤后功能恢复的效果。方法雄性Sprague-Dawley 大鼠30 只建立面神经挤压损伤模型，20 只纳入实验，随机分为治疗组和对照组各10 只。治疗组局部给予胰岛素250 ng/kg 注射，对照组局部给予等量生理盐水注射。于损伤后7 d 内每天进行神经功能评分；术后7 d 利用逆行荧光金标记技术观察神经纤维的再生。结果术后5~7 d，治疗组神经损伤评分均低于对照组(P<0.05)。治疗组损伤侧面神经核内逆行荧光金标记的

关键词: 面神经挤压伤, 面肌, 神经功能, 神经再生, 胰岛素, 大鼠

Abstract: Objective To investigate the effect of local insulin injection on neurological function after crushed injury of facial nerve in rats. Methods Facial nerve was crushing injured in 30 Sprague-Dawley rats, from which 20 selected rats were divided into treatment group (n=10) and control group (n=10). The treatment group was injected with insulin 250 ng/kg at innervated muscle, and the control group was injected with normal saline. They were assessed with neurologic deficit score everyday within 7 days after injection, and the regeneration of nerve fiber was observed with retrograde fluorogold labeling. Results Compared with that in the control group, the neurological deficit score was improved more 5~7 days after injection in the treatment group, and the count of fluorogold labeling cells increased more in the treatment group. Conclusion Local injection of insulin can promote the recovery of neurological function after crushed injury of facial nerve.

Key words: facial nerve crushed injury, facial muscle, neurological function, nerve regeneration, insulin, rats