《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (06): 642-647.

• 特稿 • 上一篇    下一篇

电针百会穴对APP/PS1双转基因小鼠学习记忆及脑源性神经营养因子表达的影响

陈吉祥 1，吴羽楠 1，郑雅媗 2，卓沛元 1，张颖铮 1，陈立典 1   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-06-25 发布日期:2015-06-25

Effects of Electroacupuncture at Baihui (DU20) on Learning and Memory and Expression of Brain-derived Neurotrophic Factor in APP/PS1 Double-transgenic Mice

CHEN Ji-xiang1, WU Yu-nan1, ZHENG Ya-xuan2, ZHUO Pei-yuan1, ZHANG Ying-zheng1, CHEN Li-dian1   

  1. 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China; 2. College of Pharmacy, Fujian University of Traditional Chinese Medicene, Fuzhou, Fujian 350122, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-06-25 Online:2015-06-25

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摘要： 目的 观察电针百会穴对 APP/PS1双转基因小鼠学习记忆功能及脑源性神经营养因子(BDNF)表达的影响，探讨电针治疗阿尔茨海默病的作用机制。方法 将 30只 4月龄雌性 APP/PS1双转基因小鼠随机分为模型组、百会组和非穴组，每组 10只，另取10只同窝阴性野生小鼠为野生组。百会组电针百会穴，非穴组电针非穴，干预28 d，野生组和模型组不干预。采用Morris水迷宫实验观察小鼠学习记忆能力；免疫组化技术观察小鼠大脑皮质β-淀粉样蛋白的沉积；Western blotting和RT-PCR法检测小鼠皮质脑源性神经营养因子(BDNF)蛋白和基因的表达情况。结果 与模型组相比，百会组小鼠逃避潜伏期缩短(P<0.05)，跨越平台次数明显增加(P<0.01)；β-淀粉样蛋白的沉积减少(P<0.05)；BDNF蛋白和基因的表达明显增多(P<0.01)。非穴组与模型组相比无显著性差异(P>0.05)。结论 电针百会穴可改善APP/PS1双转基因小鼠的学习记忆功能，其作用机制可能与增加大脑皮质BDNF蛋白和基因的表达，降低β-淀粉样蛋白的沉积有关。

关键词: 电针, 百会穴, APP/PS1, 学习记忆, 脑源性神经营养因子, 小鼠

Abstract: Objective To explore the effects of electroacupuncture at Baihui (DU20) acupoint on learning and memory and its possible mechanism through the expression of brain-derived neurotrophic factor (BDNF) in APP/PS1 double-transgenic mice. Methods 30 female APP/PS1 double transgenic mice were randomly divided into model group, DU20 group and non-acupoint group, and 10 wild type mice consisted of wild group. DU20 group received electroacupuncture at Baihui and the non-acupoint group received electroacupuncture at non-acupoint for 28 days. Learning and memory was tested by Morris water maze. Deposition of β-amyloid (Aβ) peptide was determined by immunohistochemical staining. The expression of BDNF in cortex was examined by RT-PCR and Western blotting. Results Compared with the model group, DU20 group ameliorated the learning and memory ability of APP/PS1 double-transgenic mice (P<0.05), decreased the deposition of Aβ peptide (P<0.05) and upregulated the gene and protein levels of BDNF (P<0.01). There was no significant difference between the model group and non-acupoint group (P>0.05). Conclusion Electroacupuncture at DU20 acupoint could ameliorate learning and memory in APP/PS1 double-transgenic mice. The mechanism may be related to increase the expression of BDNF and decrease the deposition of Aβ.

Key words: electroacupuncture, Baihui (DU20), APP/PS1, learning and memory, brain-derived neurotrophic factor, mouse