《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (10): 1138-1144.

• 基础研究 • 上一篇    下一篇

原花青素对脑缺血再灌注损伤后肠道功能的保护作用

范加维，杨森，杨拯，黄宏达，陈勇，宋强，罗兰，梁杰，张婷，黄倩   

  1. 成都医学院，四川成都市 610500。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-10-25 发布日期:2015-10-25

Protective Effects of Proanthocyanidins on Intestinal Function after Cerebral Ischemia-reperfusion Injury

FAN Jia- wei, YANG Sen, YANG Zheng, HUANG Hong- da, CHEN Yong, SONG Qiang, LUO Lan, LIANG Jie, ZHANG Ting, HUANG Qian   

  1. Chengdu Medical College, Chengdu, Sichuan 610500, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-10-25 Online:2015-10-25

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摘要： 目的 探讨原花青素预处理对脑缺血再灌注损伤后肠道功能的保护作用。方法 将 24只 Sprague-Dawley大鼠随机分为假手术组(A组，n=8)、缺血再灌注组(B组，n=8)和原花青素预处理组(C组，n=8)。参照 Longa线栓法制备大鼠脑缺血再灌注损伤模型。C组腹腔注射原花青素10 mg/(kg &#8901; d)，A组和B组注射等量生理盐水，连续5 d。分别于术后1 d、3 d，测定大鼠回肠肌电慢波和平滑肌收缩、血清超氧化物歧化酶(SOD)活性和血清丙二醛(MDA)含量；ELISA试剂盒测定血清TNF-α含量；取回肠组织进行苏木精-伊红(HE)染色和含水量测定。结果 脑缺血再灌注 1 d、3 d后，C组肠黏膜损伤较 B组减轻，肠黏膜损伤评分降低(P<0.05)，黏膜炎性细胞浸润数目减少；C组慢波和收缩频率有升高趋势(P>0.05)，振幅较 B组均增加(P<0.05)；C组血清 SOD活力增加(P<0.05)，MDA含量和TNF-α含量明显下降(P<0.01)，肠含水量明显降低(P<0.01)。结论 原花青素预处理对脑缺血损伤后的肠道功能有保护作用。

关键词: 脑缺血再灌注, 原花青素, 肠道功能, 大鼠

Abstract: Objective To explore the protective effects of proanthocyanidins pretreatment on intestinal function after cerebral ischemia-reperfusion injury. Methods 24 Sprague-Dawley rats were randomly divided into sham group (group A, n=8), ischemia-reperfusion group (group B, n=8) and proanthocyanidins pretreatment group (group C, n=8). The model of cerebral ischemia-reperfusion injury in rats was established according to Longa's method. Group C was intraperitoneally injected with proanthocyanidins 10 mg/(kg &#8901; d), group A and group B were injected with normal saline for 5 consecutive days. 1 and 3 days after cerebral ischemia-reperfusion, ileum myoelectric slow wave and smooth muscle contractility, the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) were measured, the content of the serum TNF-α was tested with ELISA kit, ileum tissues were tested with hematoxylin eosin (HE) staining and used for measuring the moisture content. Results Compared with group B 1 and 3 days after cerebral ischemia-reperfusion, the intestinal mucosa injury relieved, the intestinal mucosa score decreased (P<0.05) and the number of infiltrated inflammatory cell decreased in group C; the frequency of slow wave and contraction trended to increase (P>0.05), and the amplitude increased (P<0.05) in group C; the serum SOD activity increased (P<0.05), and the content of MDA and TNF-α decreased (P<0.01) in group C; the intestinal moisture content reduced (P<0.01) in group C. Conclusion Proanthocyanidins pretreatment can protect intestinal function from injury after cerebral ischemia-reperfusion.

Key words: cerebral ischemia-reperfusion, proanthocyanidins, intestinal function, rats