《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (10): 1156-1161.

• 基础研究 • 上一篇    下一篇

小鼠脊髓损伤后基质金属蛋白酶-9及血脊髓屏障的变化

荆瀛黎 1-5，刘小野 6，白帆 1-5，陈惠 1-5   

  1. 1.首都医科大学康复医学院，北京市 100068；2.中国康复研究中心北京博爱医院，北京市 100068；3.中国康复科学所，北京市 100068；4.北京脑重大疾病研究院神经损伤与修复研究所，北京市 100068；5.神经损伤与康复北京市重点实验室，北京市 100068；6.首都医科大学 附属北京友谊医院，北京市 100050。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-10-25 发布日期:2015-10-25

Alteration of Blood-spinal Cord Barrier and Matrix Metalloproteinases-9 in Mice after Spinal Cord Injury

JING Ying-li1-5, LIU Xiao-ye6, BAI Fan1-5, CHEN Hui1-5   

  1. 1.Capital Medical University School of Rehabilitation Medicine, Beijing 100068; 2. Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068; 3. Institute of Rehabilitation Science of China, Beijing 100068; 4. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100068; 5. Beijing Municipal Key Laboratory for Neural Injury and Rehabilitation, Beijing 100068; 6. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-10-25 Online:2015-10-25

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摘要： 目的 探讨脊髓损伤后基质金属蛋白酶-9(MMP-9)表达变化对血脊髓屏障(BSCB)通透性的影响。方法 C57BL/6小鼠(n=68)随机分为假手术组、损伤后2 d组(S2)、损伤后7 d组(S7)和损伤后14 d组(S14)，每组17只。损伤组采用改良Allen's法制作中度脊髓损伤模型。在损伤后的第2、7、14天分别对小鼠进行盲法的小鼠后肢运动功能(BMS)评分和BMS亚评分；各组小鼠腹腔注入伊文思蓝，检测 BSCB通透性；采用免疫荧光法检测紧密连接蛋白(occludin)表达，采用免疫印迹法检测 occludin和 MMP-9表达。结果 术后 2 d BMS评分显著降低，BSCB通透性显著增高；术后 14 d BMS评分升高(P<0.01)，BSCB通透性下降(P<0.05)。术后7 d，occludin表达下调，MMP-9表达上调(P<0.05)；术后14 d occludin表达上调，MMP-9表达下调(P<0.05)。结论 脊髓损伤后MMP-9可能通过介导occludin表达，影响BSCB通透性改变。

关键词: 脊髓损伤, 血脊髓屏障, 基质金属蛋白酶-9, 紧密连接蛋白, 小鼠

Abstract: Objective To investigate the influence of matrix metalloproteinases-9 (MMP-9) on permeability of blood-spinal cord barrier (BSCB) after spinal cord injury (SCI). Methods 68 male C57BL/6 mice were randomly divided into sham group, 2 days group (S2), 7 days group (S7) and 14 days group (S14) after SCI with 17 mice in each group. All the groups received a moderate impacted spinal cord injury except the sham group. Evan's Blue (EB) was administered intraperitoneally to detect the permeability of BSCB. Occludin was analyzed by immunofluorescence, the expressions of occludin and MMP-9 were detected by Western blotting. Results After SCI, BMS score significantly reduced, compared with S2 group, S14 group showed a significant increase (P<0.01). The permeability of BSCB was seriously damaged after SCI. Compared with S2 group, S14 group showed a notable down-regulation in the permeability of injured micro-vessels (P<0.05). The expression of occludin was down-regulated and the expression of MMP-9 was up-regulated 7 days after SCI (P<0.05). Compared with S2 group, S14 group showed a significant up-regulation of occludin and a remarkable down-regulation of MMP-9 (P<0.05). Conclusion After SCI, MMP-9 might mediate the expression of occludin to influence the BSCB permeability.

Key words: spinal cord injury, blood-spinal cord barrier, matrix metalloproteinases-9, occludin, mice