《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (11): 1241-1244.

• 国际会议报道 •    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠血管内皮生长因子和成纤维生长因子-2表达的影响

郭德玉 1，孙芳玲 1，魏仁平 2，刘婷婷 1，程华 1，艾厚喜 1，田欣 1，祝自新 1，郑文荣 1，王宇峰 1，王文 1,3
  

  1. 作者单位：1.首都医科大学宣武医院实验动物室，北京市老年病医疗研究中心，北京市 100053；2. 河北北方学院，河北张家口市 075000；3.北京市脑重大疾病研究院，北京市 100069。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-11-25 发布日期:2015-11-25

Effects of Morroniside on Expression of Vascular Endothelial Growth Factor and Fibroblast Growth Factor-2 in Rats after Focal Cerebral Ischemia-reperfusion

GUO De-yu1, SUN Fang-ling1, WEI Ren-ping2, LIU Ting-ting1, CHENG hua1, AI Hou-xi1, TIAN Xin1, ZHU Zi-xin1, ZHENG Wen-rong1, WANG Yu-feng1, WANG Wen1,3
  

  1. 1. Xuanwu Hospital, Capital Medical University, Beijing 100053, China; 2. Hebei North University, Zhangjiakou, Hebei 075000, China; 3. Beijing Institute for Brain Disorders, Beijing 100069, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-11-25 Online:2015-11-25

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被引次数

3

摘要： 目的 观察莫诺苷对局灶性脑缺血再灌注大鼠皮层血管内皮生长因子(VEGF)和成纤维生长因子-2 (FGF-2)表达的影响。方法 25只成年雄性 Sprague-Dawley大鼠随机分为假手术组、模型组、莫诺苷小剂量组(30 mg/kg)、莫诺苷中剂量组(90 mg/kg)和莫诺苷大剂量组(270 mg/kg)。采用改良线栓法制备大鼠大脑中动脉栓塞(MCAO) 30 min再灌注模型。再灌注7 d后利用蛋白免疫印迹法检测梗死侧皮层 VEGF 和 FGF-2 蛋白表达。结果 与假手术组相比，缺血再灌注 7 d 后，各组梗死侧大脑皮层 VEGF、FGF-2的表达均有增加(P<0.05)；莫诺苷小、中、大剂量组 VEGF表达进一步增加(P<0.05)；莫诺苷大剂量组 FGF-2的表达进一步显著增加(P<0.001)。结论 莫诺苷可以促进局灶性脑缺血再灌注后 VEGF和 FGF-2的表达，可能有助于促进缺血性脑损伤后的血管新生。

关键词: 脑缺血再灌注, 莫诺苷, 血管新生, 血管内皮生长因子, 成纤维生长因子-2, 大鼠

Abstract: Objective To investigate the effects of morroniside on the expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) in rat cortex after focal cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were randomly divided into sham group, model group, morroniside- low group (30 mg/kg), morroniside- middle group (90 mg/kg) and morroniside-high group (270 mg/kg). Middle cerebral arteries of rats were occluded for 30 minutes with Longa's method and re-perfused. The expression of VEGF and FGF-2 in the ischemic ipsilateral cortex was detected with Western blotting 7 days after reperfusion. Results The expression of both VEGF and FGF-2 increased in the ischemic ipsilateral cortexin in all the ischemic groups compared with the sham group (P<0.05). The expression of VEGF further increased in a dose-dependent manner in all the morroniside groups compared with that of model group (P<0.05), and the expression of FGF-2 increased in the morroniside-high group (P<0.001). Conclusion Morroniside could increase the expression of VEGF and FGF-2 after ischemia-reperfusion, which might promote angiogenesis.

Key words: cerebral ischemia-reperfusion, morroniside, angiogenesis, vascular endothelial growth factor, fibroblast growth factor-2, rats