《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (11): 1267-1272.

• 国际会议报道 • 上一篇    下一篇

脊髓损伤大鼠的学习记忆功能与海马病理改变

冯东亮 1,2，南伟 1,2，伍亚民 3，王莉 3，江龙 3，周开升 1,2，朱彦东 1,2，汪静 1,2，洪有建 3，龙在云 3，张海鸿 1,2
  

  1. 作者单位：1.兰州大学第二临床医学院骨科，甘肃兰州市 730030；2.甘肃省骨关节疾病研究重点实验室，甘肃兰州市 730030；3.第三军医大学大坪医院野战外科研究所三室，创伤烧伤与复合伤国家重点实验室，重庆市 400042。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-11-25 发布日期:2015-11-25

Learning and Memory Impairment and Pathology in Hippocampus in Rats with Spinal Cord Injury

FENG Dong-liang1,2, NAN Wei1,2, WU Ya-min3, WANG Li3, JIANG Long3, ZHOU Kai-sheng1,2, ZHU Yan-dong1,2, WANG Jing1,2, HONG You-jian3, LONG Zai-yun3, ZHANG Hai-hong1,2
  

  1. 1. Department of Orthopedics, Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China; 2. Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China; 3. Department 3 of Research Institute of Surgery, Daping Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing 400042, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-11-25 Online:2015-11-25

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被引次数

2

摘要： 目的 探究脊髓损伤后大鼠学习记忆功能与海马病理变化特点及关系。方法 36只成年雌性 Sprague-Dawley大鼠随机分为假手术组(n=18)和损伤组(n=18)。用Allen法打击T10脊髓制备脊髓损伤模型，打击强度为10 g×25 mm。损伤后1 d、3 d及1~5周每周1次行BBB后肢功能评分；术后5周时检测运动诱发电位，行Morris水迷宫测试；术后1周、3周、5周各组分别取4只大鼠行HE染色，检测海马细胞形态变化。结果 术后每个时间点，损伤组BBB评分均低于假手术组(P<0.05)。术后5周，损伤组运动诱发电位N1、P1波潜伏期显著长于假手术组(P<0.001)，振幅显著低于假手术组(P<0.001)。Morris水迷宫测试，损伤组到达平台潜伏期显著长于假手术组(P<0.001)，在目标象限的探索时间显著少于假手术组(P<0.001)，且多以系统定位或环形定位方法寻找目标平台，而假手术组则以空间定位方法为主。HE染色显示，1周时，损伤组海马组织有少量细胞形态异常；随着时间延长，海马内形态异常细胞逐渐增多，存活细胞逐渐减少；假手术组HE染色基本正常。结论 脊髓损伤可引起大鼠学习记忆功能障碍，可能与海马细胞损伤有关。

关键词: 脊髓损伤, 学习记忆功能, 海马, 病理, 大鼠

Abstract: Objective To explore the learning and memory impairment and pathology in hippocampus in rats after spinal cord contusion. Methods 36 adult female Sprague-Dawley rats were randomly divided into sham group (n=18) and spinal cord injury group (n=18). Spinal cord injury model at T10 was established with modified Allen's technique (10 g×25 mm). The hindlimbs behavior of rats was rated with Basso-Beattie-Bresnahan (BBB) scores once a week for 5 weeks. They were tested with motor evoked potentials (MEP) and Morris water maze 5 weeks after injury. The pathology of hippocampus was detected with HE staining 1 week, 3 weeks and 5 weeks after injury, 4 rats in a group, repectively. Results The BBB scores were significantly lower in the spinal cord injury group than in the sham group at each time point (P<0.05). The latencies of both N1 and P1 wave of MEP were significantly longer in the spinal cord injury group than in the sham group (P<0.001), while the amplitudes were significantly less (P<0.001). For the Morris water maze, the latency of arrival platform were significantly longer in the spinal cord injury group than in the sham group (P<0.001), and the time in target was significantly less (P<0.001), with more systematic positioning or annular positioning, while the sham group with more space-based positioning. Morphologically abnormal cells in hippocampus gradually increased since the first week after injury, with the decrease of cells survival, while it was normal in the sham group. Conclusion Spinal cord contusion can cause learning and memory impairment in rats, which may be related to injury in hippocampus.

Key words: spinal cord injury, learning and memory, hippocampus, pathology, rats