《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (12): 1379-1384.

• 国际会议报道 • 上一篇    下一篇

通窍活血汤对脑外伤大鼠认知功能障碍的影响

孙新亭 1,2，迟茜茜 1,2，孙晓静 2
  

  1. 1.中国康复研究中心北京博爱医院，北京市 100068；2.首都医科大学康复医学院，北京市 100068。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-12-25 发布日期:2015-12-25

Effect of Tongqiaohuoxue Decoction on Cognitive Impairment in Rats with Traumatic Brain Injury

SUN Xin-ting1,2, CHI Qian-qian1,2, SUN Xiao-jing2
  

  1. 1.Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China; 2. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-12-25 Online:2015-12-25

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摘要： 目的 观察通窍活血汤对脑外伤大鼠认知功能的影响并探索其作用机制。方法 70只雄性 Sprague-Dawley大鼠随机分为正常组(n=10)、假手术组(n=20)、自然恢复组(n=20)和治疗组(n=20)。采用控制性皮质撞击的方法制作大鼠脑外伤模型。治疗组大鼠在造模后每只鼠平均每天饮通窍活血汤 5 ml，其他组每天饮用蒸馏水，持续 4周。术后 1、2、3、4周对大鼠进行 Morris水迷宫测试，免疫组化检测海马区脑源性营养因子(BDNF)和突触素(SynⅠ)的表达。结果 自然恢复组各时间点逃避潜伏期较正常组增加(P<0.05)，治疗组 1、2周较正常组增加(P<0.05)，3、4周无显著性差异(P>0.05)；治疗组 3、4周小于自然恢复组(P<0.05)。自然恢复组各时间点游泳时间百分比较正常组降低(P<0.05)；治疗组 1、2、3周较正常组降低(P<0.05)，4周时无显著性差异(P>0.05)；治疗组4周时游泳时间百分比高于自然恢复组(P<0.05)。自然恢复组1、2周时海马区BDNF表达低于正常组(P<0.05)，3、4周时无显著性差异(P>0.05)，治疗组 1、2、3周与正常组无显著性差异(P>0.05)，4周时明显高于正常组(P<0.01)；治疗组在 2、3、4周海马区BDNF表达高于自然恢复组(P<0.05)。自然恢复组各时间点SynⅠ表达显著低于正常组(P<0.001)，治疗组1、2、3周明显低于正常组(P<0.01)，4周时无显著性差异(P>0.05)；治疗组3、4周明显高于自然恢复组(P<0.01)。结论 通窍活血汤能够改善脑外伤大鼠的认知功能，其机制可能是通过影响海马区的神经重塑。

关键词: 脑外伤, 认知障碍, 通窍活血汤, 大鼠

Abstract: Objective To observe the effect of Tongqiaohuoxue decoction on cognitive impairment in rats with traumatic brain injury and explore the mechanisms. Methods 70 Sprague-Dawley rats were randomized into normal group (n=10), sham operated group (n=20), untreated group (n=20) and treatment group (n=20). Controlled cortical impact device was utilized to establish traumatic brain injury model. The treatment group received Tongqiaohuoxue decoction 5 ml/d, and other groups received distilled water. The cognitive function of rats was evaluated with Morris water maze 1, 2, 3, 4 weeks post operation. And the rats were sampled to test the expression of brain-derived neurotrophic factor (BDNF) and synaptophysin (Syn) I in hippocampus through immunohistochemistry. Results The escape latency was longer in the untreated group than in the normal group in all the time points (P<0.05), and was longer in the treatment group than in the normal group 1 and 2 weeks after injury (P<0.05), with no significant difference 3 and 4 weeks after injury (P>0.05). And it was shorter in the treatment group than in the untreated group 3 and 4 weeks after injury (P<0.05). The percentage of swimming time was lower in the untreated group than in the normal group in all the time points (P<0.05), and was lower in the treatment group than in the normal group 1, 2, and 3 weeks after injury (P<0.05), with no significant difference 4 weeks after injury (P>0.05). And it was higher in the treatment group than in the untreated group weeks after injury (P<0.05). The BDNF expression was lower in the untreated group than in the normal group 1 and 2 weeks after injury (P<0.05), with no significant difference 3 and 4 weeks after injury (P>0.05). It was higher in the treatment group than in the normal group 4 weeks after injury (P<0.01), and was higher in the treatment group than in the untreated group 2, 3 and 4 weeks after injury (P<0.05). The Syn I expression was lower in the untreated group than in the normal group in all the time points (P<0.001), and was lower in the treatment group than in the normal group 1, 2 and 3 weeks after treatment (P<0.01). And it was higher in the treatment group than in the untreated group 3 and 4 weeks after injury (P<0.01). Conclusion Tongqiaohuoxue decoction could improve the cognitive function in rats with traumatic brain injury. The change in expression of BDNF and Syn I might be associated with the improvements.

Key words: traumatic brain injury, cognitive impairment, Tongqiaohuoxue decoction, rats