《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (12): 1397-1401.

• 国际会议报道 • 上一篇    下一篇

葡萄籽原花青素通过细胞外信号调节激酶1/2途径对放射性脑损伤大鼠海马区生长相关蛋白-43活性的影响

肖颖 1，刘永亮 2
  

  1. 1.唐山市工人医院，河北唐山市 063000；2.唐山市人民医院，河北唐山市 063000。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-12-25 发布日期:2015-12-25

Effect of Grape Seed Proanthocyanidin on Growth Associated Protein-43 through Extracellular Signal Regulated Kinase 1/2 Pathway in Rats with Brain Radiation Injury

XIAO Ying1, LIU Yong-liang2
  

  1. 1.Tangshan Worker Hospital, Tangshan, Hebei 063000, China; 2. Tangshan People's Hospital, Tangshan, Hebei 063000, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-12-25 Online:2015-12-25

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摘要： 目的 观察葡萄籽原花青素(GSPE)对放射性脑损伤大鼠的防治作用，并分析可能的保护机制。方法 72只3月龄雄性Sprague-Dawley大鼠随机分为对照组(n=18)、模型组(n=18)、低剂量 GSPE组(n=18)和高剂量 GSPE组(n=18)。用直线加速器进行脑部照射22 Gy制作放射性脑损伤模型。采用穿梭箱实验检测大鼠学习能力；HE染色观察海马区神经细胞组织形态变化；RT-PCR法检测生长相关蛋白-43(GAP-43) mRNA表达，Western blotting检测磷酸化的细胞外信号调节激酶 1/2 (ERK1/2)表达。结果 与模型组比较，各 GSPE组动物穿梭箱主动回避反应率显著升高(P<0.001)，被动回避潜伏期显著缩短(P<0.001)；海马区神经细胞结构损伤减轻；GAP-43 mRNA和磷酸化 ERK1/2表达水平增高(P<0.001)。且高剂量 GSPE组显著优于低剂量 GSPE组(P<0.001)。模型组中 GAP-43 mRNA表达水平与磷酸化 ERK1/2水平呈正相关(r=0.764, P<0.001)；低剂量 GSPE组和高剂量 GSPE组中 GAP-43 mRNA表达水平与磷酸化 ERK1/2水平呈正相关(r=0.814, 0.822, P<0.001)。结论 GSPE通过 ERK1/2途径提高大鼠海马区 GAP-43表达，发挥防治放射性脑损伤作用。

关键词: 放射性脑损伤, 学习, 生长相关蛋白-43, 细胞外信号调节激酶1/2, 大鼠

Abstract: Objective To observe the effect of grape seed proanthocyanidin extract (GSPE) on growth associated protein-43 (GAP-43) through extracellular signal regulated kinase 1/2 (ERK1/2) pathway in rats with brain radiation injury. Methods 72 3- month male Sprague-Dawley rats were divided into control group (n=18), model group (n=18), high dose GSPE group (n=18) and low dose GSPE group (n=18). The brain radiation injury models were established by linear accelerator irradiation with 22 Gy. The learning ability was assessed with shuttle box. The morphological changes of neurons in hippocampus were observed with HE staining; the expression of GAP-43 was detected by RT- PCR; and the expression of phosphorylated ERK1/2 was detected by Western blotting. Results Compared with the model group, the active avoidance reaction rate in the shuttle box test increased and the passive avoidance latency shortened in GSPE groups (P< 0.001); the nerve cell morphological injury reduced and the expression of GAP-43 mRNA and phosphorylated ERK1/2 increased in the GSPE groups (P<0.001), especially in the high dose GSPE group (P<0.001). The GAP-43 mRNA level positively correlated with phosphorylated ERK1/2 level in the model group (r=0.764, P<0.001), the low dose GSPE group (r=0.814, P<0.001) and the high dose GSPE group (r= 0.822, P<0.001). Conclusion GSPE could promote the expression of GAP-43 through ERK1/2 pathway in rats, and prevent the brain from radiation injury.

Key words: brain radiation injury, learning, growth associated protein-43, extracellular signal regulated kinase 1/2, rats