《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

莫诺苷对局灶性脑缺血再灌注大鼠皮层CD34表达的影响①

王志敏1,2，孙芳玲1，刘婷婷1，程华1，向本旭1，魏仁平1，艾厚喜1，田欣1，祝自新1，郑文荣1，王宇峰1，郭德玉1，王文1,3   

  1. 1.首都医科大学宣武医院实验动物室，北京市老年病医疗研究中心，北京市100053；2.曲靖医学高等专科学校，云南曲靖市655000；3.北京市脑重大疾病研究院，北京市100069。
  • 出版日期:2016-01-25 发布日期:2016-05-23

Effects of Morroniside on Expression of CD34 in Ipsilateral Cortex of Focal Cerebral Ischemia-reperfusion Rats

WANG Zhi-min1,2, SUN Fang- ling1, LIU Ting- ting1, CHENG Hua1, XIANG Ben- xu1, WEI Ren- ping1, AI Hou-xi1, TIAN Xin1, ZHU Zi-xin1, ZHENG Wen-rong1, WANG Yu-feng1, GUO De-yu1, WANG Wen1,3   

  1. 1. Department of Animal Laboratory, Xuanwu Hospital of Capital Medical University, Beijing Geriatrics Medical Research Center, Beijing 100053, China; 2. Qujing Medical College, Qujing, Yunnan 655000, China; 3. Beijing Institute for Brain Disorders, Beijing 100069, China
  • Published:2016-01-25 Online:2016-05-23

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摘要： 目的观察莫诺苷对局灶性脑缺血再灌注大鼠皮层CD34表达的影响。方法45 只健康成年雄性Sprague-Dawley 大鼠分为假手术组，模型组，莫诺苷小、中、大剂量组，每组9 只。线栓法制备大鼠大脑中动脉阻塞30 min 后再灌注模型。术后莫诺苷小、中、大剂量组予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天灌胃1 次。7 d 后采用免疫组化(n=6)及Western blotting 法(n=3)观察缺血侧皮层CD34表达。结果模型组大鼠患侧皮层CD34表达显著升高，莫诺苷大剂量组较模型组显著升高(F>14.865, P<0.001)。结论莫诺苷可能通过上调局灶性脑缺血再灌注大鼠皮层CD34的表达，促进血管新生。

关键词:  , 脑缺血再灌注, 莫诺苷, CD34, 血管新生

Abstract: Objective To explore the effects of morroniside on the expression of CD34 in ipsilateral cortex of rats after focal cerebral ischemia- reperfusion. Methods 45 male Sprague- Dawley rats were divided into sham group (n=9), ischemia group (n=9), and morroniside groups (low, medium and high dosage groups, n=9). The middle cerebral artery were occluded for 30 minutes, and reperfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg, 270 mg/kg after operation. The expression of CD34 in the ischemic ipsilateral cortex were detected with immunohistochemistry (n=6) and Western blotting (n=3) 7 days after operation. Results The expression of CD34 increased in the ischemia group compared with the sham group, and further increased in the morroniside groups of high dosage compared with the ischemia group (F>14.865, P<0.001). Conclusion Morroniside could increase the expression of CD34 in the ischemic ipsilateral cortex after ischemia-reperfusion in rats, which may promote the angiogenesis and neurogenesis after ischemia.

Key words: cerebral ischemia-reperfusion, morroniside, CD34, angiogenesis