《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

深刺加电针对兔膝骨关节炎软骨的影响①

付妮妮1，李学智1，刘菲1，席小芳1，任毅2，杨晓光1，张愉2   

  1. 1.重庆医科大学中医药学院，重庆市400016；2.汉中市中心医院，陕西汉中市723000。
  • 出版日期:2016-01-25 发布日期:2016-05-23

Effects of Deep Electroacupuncture on Cartilage in Knee Osteoarthritis Rabbits

FU Ni-ni1, LI Xue-zhi1, LIU Fei1, XI Xiao-fang1, REN Yi2, YANG Xiao-guang1, ZHANG Yu2   

  1. 1. College of Traditionl Chinese Medicine, Chongqing Medical University, Chongqing 400016, China; 2. Central Hospital of Hanzhong, Hanzhong, Shaanxi 723000, China
  • Published:2016-01-25 Online:2016-05-23

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摘要： 目的观察深刺加电针对兔膝骨关节炎软骨组织的影响。方法新西兰兔40 只随机分为正常组(A组)10 只、造模组30只，后者以Hulth-Telhag 法复制兔左膝骨关节炎模型，X线评价造模情况。造模成功后随机分为模型组(B 组, n=10)、深刺加电针组(C 组, n=10)和普通电针组(D 组, n=10)，造模后第6 周开始对C组和D组进行治疗，共4 周。治疗结束后检测关节液pH值，透射电镜下观察软骨细胞组织结构和病理学改变，检测软骨细胞凋亡指数，Western blotting 检测软骨组织中酸离子敏感通道1(ASIC1)、p38 丝裂原活化蛋白激酶(p38MAPK)的磷酸化水平和p53 蛋白表达，免疫组织化学法检测软骨组织中ASIC1 分布。结果关节液中pH值从高到低依次为A组=C组>D组>B组(P<0.01)； A组、C组、D组膝关节软骨组织形态较B组完整，C组凋亡率低于B组和D组(P<0.01)，与A组无显著性差异(P>0.05)；软骨组织中ASIC1 和p53 表达由低到高均为A组=C组

关键词: 膝骨关节炎, 电针, 深刺, 凋亡, 酸离子敏感通道1, p38 丝裂原活化蛋白激酶, p53, 兔

Abstract: Objective To observe the effects of deep electroacupuncture on carlilage tissue in knee osteoarthritis (KOA) rabbits. Methods 40 New Zealand rabbits were randomly divided into normal group (A, n=10) and model group (n=30). The model group was modeled KOA with Hulth-Telhag way, and identified with X-ray. Then they were divided into no-treated group (B, n=10), deep electroacupuncture group (C, n=10) and routine electroacupuncture group (D, n=10) randomly. The groups C and D accepted electroacupuncture since 6 weeks after modeling, for 4 weeks. They were measured with pH of joint fluid, observed structure and pathology of cartilage under transmission electron microscope, detected apoptosis index, and determined the expression of acid-sensing ion channel 1 (ASIC1), p38 mitogen-activated protein kinases (p38MAPK) and p53 with Western blotting, and distribution of ASIC1 with immunohistochemistry in cartilage tissue. Results The pHs of joint fluid from high to low were ranged as the groups A=C>D>B (P<0.01). The cartilage structure was more complete in the groups A, C and D than in the group B. The apoptosis rates from less to more were ranged as the groups A=C

Key words: knee osteoarthritis, electroacupuncture, deep acupuncture, apoptosis, acid-sensing ion channel 1, p38 mitogen-activated protein kinases, p53, rabbits