《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

酸性成纤维细胞生长因子保护庆大霉素对海马星形胶质细胞毒性作用的分裂原活化蛋白激酶通路机制①

沈丽1，黄巨恩1，黄太萍1，沈慧1，李校堃2   

  1. 1.广西医科大学护理学院，广西南宁市530021；2.温州医学院生物与天然药物研究院，浙江温州市325027。
  • 出版日期:2016-03-25 发布日期:2016-06-01

Effects of Acidic Fibroblast Growth Factor on Hippocampal Astrocytes Injury Induced by Gentamicin: Role of Mitogen-Activated Protein Kinase Signal Pathway

SHEN Li1, HUANG Ju-en1, HUANG Tai-ping1, SHEN Hui1, LI Xiao-kun2   

  1. 1. School of Nursing, Guangxi Medical University, Nanning, Guangxi 530021, China; 2. Institute of Biological and Natural Medicine ofWenzhou Medical College,Wenzhou, Zhejiang 325027, China
  • Published:2016-03-25 Online:2016-06-01

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摘要： 目的探讨酸性成纤维细胞生长因子(aFGF)对庆大霉素损伤的海马星形胶质细胞保护作用可能的机制。方法新生24 h Sprague-Dawley 大鼠分离、纯化海马星形胶质细胞，胶质纤维酸性蛋白(GFAP)免疫荧光染色鉴定，传3 代细胞接种于24 孔培养板培养3 d，分为3 组：对照组正常培养，损伤组以2.0 g/L 庆大霉素培养24 h，保护组加入4.25 μg/L aFGF培养24 h 后再加入2.0g/L 庆大霉素培养24 h。Western blotting 检测P38、细胞外信号调节蛋白激酶(ERK)1、ERK2、c-Jun 氨基末端激酶(JNK)1、JNK2 的表达。结果成功培养细胞，纯度>95%。与对照组比较，损伤组ERK1 表达增加(P<0.05)；保护组与损伤组比较，P38 表达增加(P<0.05)，ERK1 表达减少(P<0.05)；其余两两比较均无显著性差异(P>0.05)。结论信号通路中的P38 和ERK1 可能在aFGF对抗庆大霉素诱导的海马星形胶质细胞损伤过程中发挥作用。

关键词: 酸性成纤维细胞生长因子, 分裂原活化蛋白激酶, 庆大霉素, 星形胶质细胞, 海马, 大鼠

Abstract: Objective To explore the mechanism of the protection of acidic fibroblast growth factor (aFGF) for hippocampal astrocytes from injury induced by gentamicin. Methods Hippocampal astrocytes were isolated from newborn (24 hours) Sprague-Dawley rats, purified, and identified with glial fibrillary acidic protein (GFAP) immunofluorescence. The third generations were cultured for 3 days and divided into 3 groups: control group was cultured routinely, injury group was cultured with 2.0 g/L gentamicin for 24 hours, and protection group was cultured with 4.25 μg/L aFGF for 24 hours and then cultured with 2.0 g/L gentamicin for 24 hours. Western blotting was adopted to detect the expressions of P38, extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK)1/2. Results Hippocampal astrocytes were culturated successfully with the purity above 95%. The ERK1 increased in the injury group compared with the control group (P<0.05). Compared with the injury group, the p38 increased (P<0.05) and the ERK1 decreased (P<0.05) in the protection group. There was no significant difference among others (P>0.05). Conclusion The mitogen- activated protein kinase signal pathway, especially P38 and ERK1, may associate with the protection of aFGF for hippocampal astrocytes from injury induced by gentamicin.

Key words: acidic fibroblast growth factor, mitogen-activated protein kinase, gentamicin, astrocytes, hippocampus, rats