《中国康复理论与实践》

《中国康复理论与实践》

• 础基研究 • 上一篇    下一篇

血清磷酸化高分子量神经微丝蛋白与大鼠脊髓损伤程度的相关性①

何件根1，李建军2,3，杨明亮2,3，武亮2,3，杨德刚2,3   

  1. 1.北京小汤山医院，北京市102211；2.首都医科大学康复医学院，北京市100068；3.中国康复研究中心北京博爱医院，北京市100068。
  • 出版日期:2016-03-25 发布日期:2016-06-01

Correlation between Serum Phosphorylated High-molecular-weight Neurofilament and Severity of Spinal Cord

HE Jian-gen1, LI Jian-jun2,3, YANG Ming-liang2,3, WU Liang2,3, YANG De-gang2,3   

  1. 1. Beijing Xiaotangshan Hospital, Beijing 102211, China; 2. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China; 3. Beijing Bo'ai Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Published:2016-03-25 Online:2016-06-01

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摘要： 目的研究血清磷酸化高分子量神经微丝蛋白(pNF-H)在脊髓损伤动物模型中的动态变化规律及其与脊髓损伤程度的相关性。方法20 只Sprague-Dawley 大鼠等分为空白对照(A)组及脊髓损伤轻(B)、中(C)、重(D)度组，检测大鼠血清pNF-H水平、BBB评分及残存白质面积百分比。结果B、C、D组大鼠血清pNF-H在术后12 h 及3 d 出现两个高峰；3 组间有显著性差异(P<0.05)；损伤后3 d 血清pNF-H水平与14 d 时BBB评分、残存白质面积百分比呈负相关性(r=-0.987, r=-0.978)。结论脊髓损伤后大鼠血清pNF-H水平存在双峰现象；脊髓损伤程度越重，大鼠pNF-H水平越高；pNF-H水平可以预测脊髓损伤严重程度。

关键词: 脊髓损伤, 神经微丝蛋白, 分子生物学评估, 大鼠

Abstract: Objective To investigate the level of phosphorylated high-molecular-weight neurofilament (pNF-H) after spinal cord injury (SCI), and explore the relationship between pNF-H and severity of SCI. Methods 20 Sprague-Dawley rats were equally divided into control group (group A), mild SCI group (group B), moderate SCI group (group C) and severe SCI group (group D). The level of pNF-H in serum, BBB score and remaining area of white matter were obtained at different time points. Results The level of serum pNF-H in groups B, C and D arrived at peaks 12 hours and 3 days after SCI, and there was significant difference among them (P<0.05). Both BBB score and remaining area of white matter 14 days after SCI negatively correlated with the level of pNF-H 3 days after SCI (r=-0.987 and r=-0.978, respectively). Conclusion The pNF-H increases twice after SCI in rats, and may be associated with the severe of SCI, which can be considered as a biomarker.

Key words: spinal cord injury, neurofilament, molecular biological evaluation, rats