《中国康复理论与实践》

《中国康复理论与实践》

• 临床研究 • 上一篇    下一篇

核苷酸结合寡聚化结构域样受体蛋白3 在阿尔茨海默病和血管性痴呆患者中的表达及相关因素研究①

柏华1a,1b，杨波1c，余德军1a，张启芳2   

  1. 1.贵州医科大学第三附属医院，a.医学实验中心；b.神经内科；c.精神科，贵州都匀市558000；2.贵州医科大学医学分子生物学重点实验室，贵州贵阳市555000。
  • 出版日期:2016-03-25 发布日期:2016-06-01

Expression of Nucleotide-binding Oligomerization Domain Receptor Protein 3 in Patients with Alzheimer's Disease and Vascular Dementia and Related Factors

BAI Hua1a,1b, YANG Bo1c, YU De-jun1a, ZHANG Qi-fang2   

  1. 1. a. Medical Laboratory Center; b. Department of Neurology; c. Department of Psychiatry; the Third Affiliated Hospital of Guizhou Medical University, Duyun, Guizhou 558000, China; 2. Key laboratory of Medical Molecular Biology, Guizhou Medical University, Guiyang, Guizhou 555000, China
  • Published:2016-03-25 Online:2016-06-01

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摘要： 目的探讨核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)对痴呆发病的影响。方法2014 年1 月~2015 年10 月，用RT-PCR方法检测16 例阿尔茨海默病(AD)患者、22 例血管性痴呆(VD)患者和20 名年龄相似的健康体检者外周血单个核细胞中NLRP3mRNA水平；ELISA法检测血清中白细胞介素(IL)-1β和IL-18 蛋白水平；收集患者外周血白细胞计数(WBC)和血清总钙浓度等临床检测资料；将NLRP3 mRNA水平分别与IL-1β、IL-18、WBC和钙浓度进行单因素相关分析。结果NLRP3 mRNA水平由高到低依次为AD组>VD组>健康组(q>11.48, P<0.05)；IL-1β水平AD组高于健康组(q=16.74, P<0.05)，AD组与VD组、VD组与健康组无显著性差异(P>0.05)；IL-18 水平各组间均无显著性差异(P>0.05)。AD组中，NLRP3 mRNA水平与IL-1β水平(r=0.64)、血清钙水平(r=0.58)呈正相关，与IL-18、WBC无相关性(P>0.05)。结论NLRP3 炎症小体中相关蛋白或基因表达与AD的发病可能有较大关系，与VD的发病关系不大。

关键词: 阿尔茨海默病, 血管性痴呆, 核苷酸结合寡聚化结构域样受体蛋白3, 炎症, 发病

Abstract: Objective To explore the role of nucleotide-binding oligomerization domain receptor protein 3 (NLRP3) inflammasome in the pathogenesis of dementia. Methods 16 patients with with Alzheimer's disease (AD), 22 patients with vascular dementia (VD) and 20 healthy persons with similar age were detected the level of NLRP3 mRNA with RT-PCR in peripheral blood mononuclear cells January, 2014 to October, 2015. The serum level of interleukin (IL)-1β and IL-18 were detected with ELISA. The total number of white blood cells (WBC) and serum total calcium were collected. Single factor correlation analysis was performed between NLRP3mRNA and IL- 1β, or IL-18, or WBC, or calcium concentration. Results The levels of NLRP3 mRNA ranged from high to low were as in AD group > VD group > the healthy group (q>11.48, P<0.05). The serum level of IL-1β was higher in AD group than in the the healthy group (q=16.74, P<0.05), but not significantly different between VD group and the healthy group, nor between VD group and AD group (P>0.05). There was no significant difference about IL-18 among AD group, VD group and the healthy group (P>0.05). NLRP3 mRNA was positively correlated with the serum levels of IL-1β (r=0.64) and calcium (r=0.58) in AD group. There was no correlation between NLRP3 mRNA and IL-18 or WBC (P> 0.05). Conclusion The expression of NLRP3 inflammasome may be related with the pathogenesis of AD, but little impact on the pathogenesis of VD.

Key words: Alzheimer's disease, vascular dementia, nucleotide- binding oligomerization domain receptor protein 3, inflammation, pathogenesis