《中国康复理论与实践》

《中国康复理论与实践》

• 础基研究 • 上一篇    下一篇

淫羊藿苷对MK-801致精神分裂症小鼠模型的影响①

陈溪，谷洪顺，张兰，李林   

  1. 首都医科大学宣武医院药物研究室，北京市老年病医疗研究中心，北京市神经药物工程技术研究中心，北京市 100053。
  • 出版日期:2016-04-25 发布日期:2016-06-01

Effects of Icariin on MK-801-induced Schizophrenia Model in Mice

CHEN Xi, GU Hong-shun, ZHANG Lan, LI Lin   

  1. Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing Engineering Reaserch Center Nerve System Drugs, Beijing 100053, China
  • Published:2016-04-25 Online:2016-06-01

PDF (PC)

摘要： 目的 研究中药淫羊藿的主要有效成分淫羊藿苷对精神分裂症动物模型的影响。方法 小鼠腹腔注射N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801(0.3 mg/kg, 0.6 mg/kg, 1.2 mg/kg)制备精神分裂症小鼠模型。应用旷场行为红外检测系统观察小鼠的活动性，确定制备精神分裂症的最佳剂量。40只小鼠随机分为正常对照组、模型组、阳性药利培酮组(0.1 mg/kg)、淫羊藿苷组(50 mg/kg)，每组 10只。阳性药利培酮组和淫羊藿苷组灌胃相应药物；除正常对照组外，其他组腹腔注射 0.1 ml MK-801溶液 0.6mg/kg造模。测定各组小鼠210 min内的活动总距离和在中心区的活动距离。结果 模型组活动总距离和中央区活动距离与正常对照组相比显著延长(P<0.001)；阳性药利培酮组两指标均短于模型组(P<0.05)；淫羊藿苷组活动总距离短于模型组，但无显著性差异(P=0.065)，中央区活动距离短于模型组(P=0.037)。淫羊藿苷组与阳性药利培酮组活动总距离和中央区活动距离比较均无显著性差异(P>0.05)。结论 淫羊藿苷能够改善精神分裂症模型小鼠的阴性症状，可能改善其阳性症状，有利于治疗精神分裂症。

关键词: 精神分裂症, 淫羊藿苷, N-甲基-D-天冬氨酸受体, MK-801, 动物模型, 旷场试验, 小鼠

Abstract: Objective To investigate the effects of icariin, a major active component of traditional Chinese herb Epimedium on schizophrenia animal model. Methods MK-801, a N-methyl-D-aspartate (NMDA) receptor antagonist, was intraperitoneally injected into mice to establish a schizophrenia animal model. The open field behavior test infrared detection system was used to measure the mobility of mice in order to find the best optimal dose. 40 mice were randomly divided in to control group, model group, risperidone group and icariin group with 10 mice in each group. The risperidone group and the icariin group were given 0.1 mg/kg risperidone and 50 mg/kg icariin respectively. All groups were injected with 0.6 mg/kg MK-801 0.1 ml intraperitoneally, except the control group. The total distance and the central distance within 210 minutes were tested. Results The total distance and the central distance were significantly longer in the model group than in the control group (P<0.001), and were shorter in the risperidone group than in the control group (P<0.05). The total distance was shorter in the icariin group than in the model group, but there was no significant difference (P=0.065); and the central distance was shorter (P= 0.037). There was no significant difference in both total distance and the central distance between the risperidone group and the icariin group (P>0.05). Conclusion Icariin may improve the positive and negative symptoms of schizophrenia mice, which may be beneficial to schizophrenia therapy.

Key words: schizophrenia, icarrin, N-methyl-D-aspartic acid receptor, MK-801, animal model, open field test, mice