《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

丙酮酸乙酯对缺氧缺血性脑损伤仔鼠海马区caspase-3表达及学习记忆能力影响 ①

曹阳 1，张丽华 2   

  1. 1.佳木斯大学康复医学院，黑龙江佳木斯市 154000；2.佳木斯大学附属第三医院脑瘫康复二科，黑龙江佳木斯市 154000。
  • 出版日期:2016-04-25 发布日期:2016-06-01

Effect of Ethyl Pyruvate on Expression of Caspase-3 in Hippocampus and Learning and Memory in Rats with Hypoxic Ischemic Brain Damage

CAO Yang1, ZHANG Li-hua2   

  1. 1. Rehabilitation College of Jiamusi University, Jiamusi, Heilongjiang 154000, China; 2. Department 2 of Cerebral Palsy, The Third Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154000, China
  • Published:2016-04-25 Online:2016-06-01

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摘要： 目的 探讨丙酮酸乙酯(EP)对新生大鼠缺氧缺血脑损伤(HIBD)后海马区caspase-3表达及学习记忆能力影响。方法 7日龄 Wistar 大鼠 90 只随机分假手术组(n=15)、HIBD 组(n=15)、EP1 组(n=15，10 ml/kg)、EP2 组(n=15，30 mg/kg)、EP3 组(n=15，50 mg/kg)和 EP4组(n=15，100 mg/kg)。采用经典 Rice法制备模型。EP各组于术前 30 min及术后每 24小时腹腔注射相应剂量 EP，共2周。免疫组化染色观察caspase-3阳性细胞数，Morris水迷宫评测逃避潜伏时间和穿过目标象限次数。结果 除EP1组caspase-3阳性细胞数与HIBD组无显著性差异(P>0.05)外，其余EP各组较HIBD组均明显减少(P<0.01)，EP3组最少(P<0.01)。Morris水迷宫测试：除 EP1组逃避潜伏期、穿过目标象限次数与 HIBD组无显著性差异(P>0.05)外，其余 EP各组均优于 HIBD组(P<0.01)，EP3组最佳(P<0.01)。结论 EP可以减少HIBD大鼠海马区caspase-3的表达，改善HIBD引起的远期学习记忆能力障碍。

关键词: 缺氧缺血性脑损伤, 丙酮酸乙酯, caspase-3, 学习记忆能力, 大鼠

Abstract: Objective To investigate the effect of ethyl pyruvate (EP) on the expression of caspase-3 in the hippocampus and the learning and memory ability in neonatal rats with hypoxic ischemic brain damage (HIBD). Methods 90 Wistar rats of 7 days old were randomly divided into sham operation group (n=15), HIBD group (n=15), EP1 group (n=15, 10 ml/kg), EP2 group (n=15, 30 mg/kg), EP3 group (n= 15, 50 mg/kg) and EP4 group (n=15, 100 mg/kg). The model was established with Rice's method. 30 minutes before operation, and every 24 hours after operation, EP groups were injected with 10 ml/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg EP in abdomen respectively, for 2 weeks. After treatment, the caspase-3 positive cells were observed by immunohistochemical staining, and the latency and the times crossing the target quadrant were tested by Morris water maze test. Results The caspase-3 positive cells were less in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). The latency and the times crossing the target quadrant were better in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). Conclusion Ethyl pyruvate can decrease the expression of caspase-3 in hippocampus, and improve the ability of memory and learning in neonatal rats with HIBD.

Key words: hypoxic ischemic brain damage, ethyl pyruvate, caspase-3, learning and memory, rats