《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

电针对阿尔茨海默病大鼠海马谷氨酸受体相互作用蛋白1、2表达的影响①

刘若兰 1，沈沉 2，沈晓燕 3，田峻 1   

  1. 1.武汉大学中南医院康复科，湖北武汉市 430071；2. 湖北省中医院针灸科，湖北武汉市 430061；3.武汉大学，湖北武汉市430071。
  • 出版日期:2016-04-25 发布日期:2016-06-01

Effects of Electroacupuncture on Expression of Glutamate Receptor Interacting Proteins 1 and 2 in Hippocampus of Rats with Alzheimer's Disease

LIU Ruo-lan1, SHEN Chen2, SHEN Xiao-yan3, TIAN Jun1   

  1. 1. Rehabilitation Department, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China; 2. Department of Acupuncture and Moxibustion, Hubei Provincial Hospital of TCM, Wuhan, Hubei 430061, China; 3. Wuhan University, Wuhan, Hubei 430071, China
  • Published:2016-04-25 Online:2016-06-01

PDF (PC)

摘要： 目的 探讨电针对Aβ25-35所致的阿尔茨海默病(AD)模型大鼠海马神经元突触后膜α-氨基-3-羟基-5-甲基-4-异唑丙(AMPA)受体相关蛋白谷氨酸受体相互作用蛋白(GRIP)1、GRIP2表达的影响。方法 40只雄性 Sprague-Dawley大鼠随机分为正常组、假手术组、模型组和电针组，每组10只。采用海马CA1区注射寡聚态Aβ25-35制备模型，假手术组在相同部位用同样方法注射等量的生理盐水。造模成功后第2天开始，电针组选取双侧肾俞、百会穴进行治疗，每天1次，每周6次，共2周。治疗结束后行免疫组化检测GRIP1、GRIP2的表达量。结果 正常组与假手术组GRIP1、GRIP2蛋白表达无显著性差异(t<1.7438, P>0.05)。与假手术组比较，模型组、电针组 GRIP1、GRIP2蛋白表达显著下降(t>9.5928, P<0.001)。与模型组比较，电针组大鼠 GRIP1、GRIP2蛋白表达升高(t>9.5326, P<0.05)。结论 电针可能通过增加与AMPA受体定位插入相关的突触后蛋白GRIP1、GRIP2的数量，使突触后膜上的AMPA受体数量增多。

关键词: 阿尔茨海默病, 电针, 谷氨酸受体相互作用蛋白, 大鼠

Abstract: Objective To investigate the effect of electroacupuncture on the expression of α-amino-3-hydroxyl-5-methyl-4 -isoxazole propionate (AMPA) receptor associated protein glutamate receptor interacting protein (GRIP)1 and GRIP2 in hippocampal neurons of rats with Alzheimer's disease (AD) model induced by Aβ25-35. Methods 40 Male Sprague-Dawley rats were randomly divided into normal group, sham operation group, model group and electroacupuncture group with 10 rats in each group. The AD rat model was prepared by injecting Aβ25-35 in the hippocampus CA1 of rats, while the sham operation group was injected with equal amount of normal saline at the same location. On the second day after successful modeling, the electroacupuncture group received electroacupuncture at Baihui (DU20) and bilateral Shenshu (BL23) acupoints, once a day, 6 times a week for 2 weeks. The expression of GRIP1 and GRIP2 were detected with immunohistochemistry. Results There was no difference in the expression of GRIP2 and GRIP1 proteins in hippocampus between the normal group and sham operation group (t<1.7438, P>0.05), but was lower in the model group and the electroacupuncture group than in the sham operation group (t>9.5928, P<0.001), and was higher in the electroacupuncture group than in the model group (t>9.5326, P<0.05). Conclusion Electroacupuncture may increase the number of AMPA receptors on the postsynaptic membrane by increasing GRIP1 and GRIP2.

Key words: Alzheimer's disease, electroacupuncture, glutamate receptor interacting protein, rats