目的 探讨针康法对脑缺血大鼠神经功能预后和细胞外信号调节激酶1/2 (ERK1/2)信号通路的影响。方法 90只雄性Sprague-Dawley大鼠随机分入假手术组(n=18)、模型组(n=18)、针刺组(n=18)、康复组(n=18)及针康组(n=18),再分为术后3 d、7 d、14 d三个亚组(n=6)。线栓法制备永久性局灶性脑缺血模型。假手术组和模型组不进行治疗,针刺组采用头穴丛刺针法治疗,康复组给予电动跑台训练,针康组采用针康法治疗。各时间点,采用改良神经损害严重程度评分进行评定,Western blotting法检测缺血半暗区皮层ERK1/2、p-ERK1/2蛋白表达。结果 术后各时间点,与模型组比较,各治疗组神经缺损评分降低(P<0.05);术后7 d、14 d,与针刺组、康复组比较,针康组评分降低(P<0.05)。术后各时间点,与模型组比较,各治疗组p-ERK1/2蛋白表达增加(P<0.05),(p-ERK1/2)/(ERK1/2)增加(P<0.05);与针刺组、康复组比较,针康组p-ERK1/2蛋白表达升高(P<0.05),(p-ERK1/2)/(ERK1/2)增加(P<0.05)。结论 针康法治疗可进一步改善脑缺血大鼠神经行为,可能与ERK1/2信号通路激活有关。
Objective To explore the effects of acupuncture-rehabilitation therapy on neurological function recovery and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway activation after permanent focal cerebral ischemia in rats. Methods Ninty male Sprague-Dawley rats were divided into five groups, namely sham group, model group, acupuncture group, rehabilitation group and acupuncture-rehabilitation group, and 18 in each group. Each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Their middle cerebral arteries were occluded except those of sham group. The sham and model groups accepted no treatment, while the acupuncture group accepted cluster needling of scalp acupuncture, rehabilitation group accepted treadmill training, and the acupuncture-rehabilitation group accepted both acupuncture and treadmill training. They were assessed with modified Neurologic Severity Score (mNSS) 3, 7 and 14 days after modeling, while the expression of ERK1/2 and p-ERK1/2 were determined with Western blotting. Results The neurologic severity score reduced in all three treatment groups (P<0.05) compared with that of the model group at every time point, and was the least in the acupuncture-rehabilitation group (P<0.05) 7 and 14 days after modeling among the treatment groups. Meanwhile, the expression of p-ERK1/2 increased in all three treatment groups (P<0.05), and was the most in the acupuncture-rehabilitation group (P<0.05), as well as the rate of (p-ERK1/2)/(ERK1/2) (P<0.05). Conclusion Acupuncture-rehabilitation therapy can promote the neurological function recovery, which may be associated with activation of ERK1/2 signaling pathway.
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