目的 研究长期丰富环境对促进小鼠缺血性脑损伤后运动、社交功能恢复的作用。方法 成年雄性ICR小鼠16只,复制大脑中动脉永久性栓塞模型。术后第1天将小鼠分为丰富环境组(n=8)和标准环境组(n=8)。术后7 d、14 d、21 d、28 d对小鼠进行改良神经损害严重程度评分(mNSS),进行疲劳转棒仪、聪明笼子检测。结果 术后28 d,丰富环境组mNSS评分与转棒疲劳试验结果优于标准环境组(t>2.927, P<0.05)。一般运动试验中,术后14~28 d,丰富环境组在聪明笼子中间区域出现的时间长于标准环境组(t>2.480, P<0.05);社交行为试验中,术后7~28 d,丰富环境组比标准环境组表现出对陌生小鼠更感兴趣的趋势,但无显著性差异(P>0.05);术后14~28 d,丰富环境组出现在中间区域的时间明显长于标准环境组(t>3.472, P<0.01);术后14 d在聪明笼子中的移动速度高于标准环境组(P<0.05)。结论 丰富环境有助于小鼠缺血性脑损伤后运动功能恢复,并可能促进其社交功能恢复。
Objective To explore the role of long-term enriched environment in promoting the recovery of motor and social function in mice after ischemic brain injury. Methods Sixteen adult male ICR mice underwent permanent middle cerebral artery occlusion (MCAO). The first day after operation, they were divided into enriched environment group (n=8) and standard condition group (n=8). The mice were tested with modified Neurological Severity Score (mNSS), rotarod test and smart cage 7, 14, 21, 28 days after modeling. Results The score of mNSS and the result of rotarod test improved more in the enriched environment group than in the standard condition group 28 days after MCAO (t>2.927, P<0.05). The occupancy time in the middle of smart cage was longer in the enriched environment group than in the standard condition group 7 to 28 days after MCAO (t>2.480, P<0.05) in the general move test. There was a trend of being more interested in strange mice in the enriched environment group than in the standard condition group, but the difference was not statistically significant (P>0.05) in the social behavior test; however, the occupancy time in the middle of smart cage was longer in the enriched environment group than in the standard condition group 14 to 28 days after MCAO (t>3.472, P<0.01), and the velocity of moving was higher 14 days after MCAO (P<0.05). Conclusion Enriched environment could promote the recovery of motor function, somehow of social function, in mice after ischemic brain injury.
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