目的探讨8 Hz、130 dB次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ(CaMKⅡ)及tau蛋白表达的影响。方法56只雄性Sprague-Dawley大鼠随机分为对照组(n=8)、1 d组(n=8)、7 d组(n=8)、14 d组(n=32)。14 d组再分为照射后1 h、6 h、24 h、48 h亚组,每亚组8只。对照组每天次声仓内无次声作用放置2 h,其余各组8 Hz、130 dB次声照射2 h。免疫组织化学、Western blotting和ELISA方法检测各组大鼠海马区磷酸化CaMKⅡ(pT286-CaMKⅡ)及tau蛋白表达。结果14 d组pT286-CaMKⅡ水平最高(F>14.912, P<0.001),tau蛋白表达最多(F>36.229, P<0.001),7 d组次之(P<0.05)。14 d组中,次声后1 h、6 h,tau蛋白表达最高,24 h有所恢复,但仍较对照组高(P<0.05)。结论8 Hz、130 dB次声作用后,大鼠海马区CaMKⅡ磷酸化水平和tau蛋白表达及磷酸化水平增高,可能参与次声致大鼠学习记忆功能受损。
ObjectiveTo study the effect of infrasound on expression of calmodulin-dependent protein kinase II (CaMKII) and tau protein in hippocampus of rats. MethodsFifty-six male Sprague-Dawley rats were randomized into control group (n=8), 1-day group (n=8), 7-day group (n=8) and 14-day group (n=32), and the 14-day group was subgrouped as 1-hour, 6-hour, 24-hour, 48-hour subgroups, naming after the time after infrasound exposure, 8 in each subgroup. All the test groups were put in an infrasound field with 8 Hz, 130 dB for 2 hours daily, while the control group was put in the infrasound instrument without infrasound exposure for 2 hours daily. The expression of pT286-CaMKII and tau protein in hippocampus was detected with immunohistochemisty, Western blotting and enzyme-linked immunoabsorbent assay. ResultsThe expression of pT286-CaMKII was the most in 14-day group (F>14.912, P<0.001), as well as the expression of tau protein (F>36.229, P<0.001), and secondary in 7-day group (P<0.05). For 14-day group, the expression of tau protein was the most in 1-hour and 6-hour subgroups, and dropped down in 24-hour subgroup, although more than that in the control group (P<0.05). ConclusionExposure of 8 Hz, 130 dB infrasound may induce phosphorylation of CaMKII and tau protein, and the expression of tau protein in hippocampal cells in rat, which may disturb their learning and memory function.
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