《中国康复理论与实践》

中国康复理论与实践 >

2017 , Vol. 23 >Issue 7: 745 - 749

DOI: https://doi.org/10.3969/j.issn.1006-9771.2017.07.001

专题脑卒中针刺机理与功能康复

电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响

  • 叶涛 ,
  • 朱路文 ,
  • 唐强 ,
  • 李宏玉 ,
  • 梁碧莹 ,
  • 张继瑶 ,
  • 郑婷婷
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  • 1.黑龙江中医药大学,黑龙江哈尔滨市 150040;
    2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001。
叶涛(1989-),男,汉族,福建漳平市人,博士研究生,主要研究方向：脑卒中中医康复基础与临床研究。

收稿日期: 2017-03-29

  网络出版日期: 2017-08-07

基金资助

1.国家自然科学基金项目(No.81503666); 2.黑龙江省自然科学基金项目(No.QC2015103); 3.哈尔滨市科技创新人才研究专项基金项目(青年后备人)(No.2014RFQGJ150); 4.黑龙江中医药大学领军人才计划项目(No.2012RCL02); 5.黑龙江中医药大学研究生创新科研项目(No.yjscx2016037)

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Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury

  • YE Tao ,
  • ZHU Lu-wen ,
  • TANG Qiang ,
  • LI Hong-yu ,
  • LIANG Bi-ying ,
  • ZHANG Ji-yao ,
  • ZHENG Ting-ting
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  • 1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China;
    2. The Second Hospital Affiliated to Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received date: 2017-03-29

  Online published: 2017-08-07

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摘要

目的探讨电针预处理对大鼠脑缺血再灌注损伤后神经功能的影响及其机制。方法雄性Sprague-Dawley大鼠36只随机分为假手术组(n=12)、模型组(n=12)和电针预处理组(n=12)。后两组线栓法制备大鼠脑缺血2 h再灌注模型。电针预处理组在缺血前给予电针百会干预2周。再灌注24 h后,采用改良神经功能缺损评分进行评定,HE染色观察缺血侧脑组织脑损伤程度,Western blotting检测脑缺血半暗区Tolls样受体4 (TLR4)、核转录因子κB (NF-κB)蛋白表达。结果与模型组比较,电针预处理组神经功能缺损评分降低(P<0.05),脑组织损伤程度减轻,缺血半暗区TLR4、NF-κB蛋白表达下调(P<0.05)。结论电针预处理通过下调缺血半暗区TLR4、NF-κB蛋白表达,诱导脑保护作用,降低炎性损伤程度,改善脑缺血再灌注损伤后大鼠的神经功能。

关键词： 脑缺血再灌注损伤; 电针; 预处理; 神经功能; Tolls样受体4; 核转录因子κ; B; 大鼠

本文引用格式

叶涛 , 朱路文 , 唐强 , 李宏玉 , 梁碧莹 , 张继瑶 , 郑婷婷 . 电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响[J]. 中国康复理论与实践, 2017 , 23(7) : 745 -749 . DOI: 10.3969/j.issn.1006-9771.2017.07.001

Abstract

ObjectiveTo investigate the effect of electroacupuncture (EA) pretreatment on neurological function, and the expression of Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) protein in ischemic penumbra after cerebral ischemia-reperfusion (CIR) injury. MethodsA total of 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and EA group (n=12). The later two groups were occluded their right middle cerebral arteries for two hours and reperfused. The EA group was pretreated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS), the injury in ischemic brain was detected with HE staining, and the expression of TLR4 and NF-κB protein in ischemic penumbra were detected with Western blotting, 24 hours after reperfusion. ResultsThe score of mNSS decreased (P<0.05), the injury of brain tissue ameliorated, and the expression of TLR4 and NF-κB decreased in ischemic penumbra (P<0.05) in EA group compared with those in the model group. ConclusionEA pretreatment can reduce the injury and improve the neurological function in rats after CIR by down-regulating the expression of TLR4 and NF-κB protein in ischemic penumbra.

Key words： cerebral ischemia-reperfusion injury; electroacupuncture; pretreatment; neurological function; Toll-like receptor 4; nuclear factor κ; B; rats

参考文献

[1] Murray CJ, Lopez AD. Measuring the global burden of disease [J]. N Engl J Med, 2013, 369(5): 448-457.
[2] Feng R, Zhang F. The neuroprotective effect of electro-acupuncture against ischemic stroke in animal model: a review [J]. Afr J Tradit Complement Altern Med, 2014, 11(3): 25-29.
[3] Liao W, Tsai T, Ho T, et al. Neuroprotective effect of paeonol mediates anti-inflammation via suppressing Toll-like receptor 2 and Toll-like receptor 4 signaling pathways in cerebral ischemia-reperfusion injured rats [J]. Evid Based Complement Alternat Med, 2016, 2016: 3704647.
[4] Liu J, Chen Q, Jian Z, et al. Daphnetin protects against cerebral ischemia/reperfusion injury in mice via inhibition of TLR4/NF-κB signaling pathway [J]. Biomed Res Int, 2016, 2016: 2816056.
[5] Wang Y, Chen G, Yu X, et al. Salvianolic acid B ameliorates cerebral ischemia/reperfusion injury through inhibiting TLR4/MyD88 signaling pathway [J]. Inflammation, 2016, 39(4): 1503-1513.
[6] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(20): 84-91.
[7] 唐强,叶涛,朱路文,等. 针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响[J]. 中国康复理论与实践, 2017, 23(1): 27-31.
[8] Li Y, Chopp M, Chen J, et al. Intrastriatal transplantation of bone marrow nonhematopoietic cells improves functional recovery after stroke in adult mice [J]. J Cereb Blood Flow Metab, 2000, 20(9): 1311-1319.
[9] Zhu L, Ye T, Tang Q, et al. Exercise preconditioning regulates the Toll-like receptor 4/nuclear factor-κB signaling pathway and reduces cerebral ischemia/reperfusion inflammatory injury: a study in rats [J]. J Stroke Cerebrovasc, 2016, 25(11): 2770-2779.
[10] Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium [J]. Circulation, 1986, 74(5): 1124-1136.
[11] Kitagawa K, Matsumoto M, Tagaya M, et al. 'Ischemic tolerance' phenomenon found in the brain [J]. Brain Res, 1990, 528(1): 21-24.
[12] Stetler RA, Leak RK, Yu G, et al. Preconditioning provides neuroprotection in models of CNS disease: paradigms and clinical significance [J]. Prog Neurobiol, 2014, 114(1): 58-83.
[13] 叶涛,朱路文,唐强. 电针预处理诱导脑缺血耐受的研究进展[J]. 针灸临床杂志, 2015, 31(2): 87-90.
[14] He X, Mo Y, Geng W, et al. Role of Wnt/β-catenin in the tolerance to focal cerebral ischemia induced by electroacupuncture pretreatment [J]. Neurochem Int, 2016, 97: 124-132.
[15] Jung YS, Lee SW, Park JH, et al. Electroacupuncture preconditioning reduces ROS generation with NOX4 down-regulation and ameliorates blood-brain barrier disruption after ischemic stroke [J]. J Biomed Sci, 2016, 23(1): 1-11.
[16] Wu ZQ, Cui SY, Zhu L, et al. Study on the mechanism of mTOR-mediated autophagy during electroacupuncture pretreatment against cerebral ischemic injury [J]. Evid Based Complement Alternat Med, 2016, 2016: 9121597.
[17] Ran QQ, Chen HL, Liu YL, et al. Electroacupuncture preconditioning attenuates ischemic brain injury by activation of the adenosine monophosphate-activated protein kinase signaling pathway [J]. Neural Regen Res, 2015, 10(7): 1069-1075.
[18] 林咸明,陈丽萍,姚旭. 不同时程电针预处理对脑缺血再灌注大鼠血脑屏障基质金属蛋白酶-9、血管内皮生长因子的影响[J]. 针刺研究, 2015, 40(1): 40-44.
[19] 朱路文,叶涛,姜云飞,等. 电针预处理对大鼠脑缺血再灌注损伤后炎性因子及细胞凋亡的影响[J]. 中国康复理论与实践, 2016, 22(7): 765-768.
[20] Zhao M, Zhou A, Xu L, et al. The role of TLR4-mediated PTEN/PI3K/AKT/NF-κB signaling pathway in neuroinflammation in hippocampal neurons [J]. Neuroscience, 2014, 269(Complete): 93-101.
[21] Raquel RD, Cristian GG, Carlos OS, et al. Calcineurin inhibitors cyclosporine A and tacrolimus induce vascular inflammation and endothelial activation through TLR4 signaling [J]. Sci Rep, 2016, 6: 27915.
[22] Li X, Yang J, Yang J, et al. RP105 protects against myocardial ischemia-reperfusion injury via suppressing TLR4 signaling pathways in rat model [J]. Exp Mol Pathol, 2016, 100(2): 281-286.
[23] Zhang J, Xia J, Zhang Y, et al. HMGB1-TLR4 signaling participates in renal ischemia reperfusion injury and could be attenuated by dexamethasone-mediated inhibition of the ERK/NF-κB pathway [J]. Am J Transl Res, 2016, 8(10): 4054-4067.
[24] Yang CJ, Yang J, Jian Y, et al. Radioprotective 105 kDa protein (RP105): Is a critical therapeutic target for alleviating ischemia reperfusion induced myocardial damage via TLR4 signaling pathway [J]. Int J Cardiol, 2016, 222: 1069-1070.
[25] Hyakkoku K, Hamanaka J, Tsuruma K, et al. Toll-like receptor 4 (TLR4), but not TLR3 or TLR9, knock-out mice have neuroprotective effects against focal cerebral ischemia [J]. Neuroscience, 2010, 171(1): 258-267.
[26] Andresen L, Theodorou K, Grünewald S, et al. Evaluation of the therapeutic potential of anti-TLR4-antibody MTS510 in experimental stroke and significance of different routes of application [J]. PLoS One, 2016, 11(2): e0148428.
[27] 于凌志,左艳丽,贾孟辉,等. 核转录因子NF-κB对脑缺血再灌注干预机制的研究进展[J]. 中国民族医药杂志, 2015, 21(7): 42-44.
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