《中国康复理论与实践》

中国康复理论与实践 >

2018 , Vol. 24 >Issue 1: 43 - 48

DOI: https://doi.org/10.3969/j.issn.1006-9771.2018.01.008

基础研究

电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响

  • 黄佳 ,
  • 宋长明 ,
  • 杨敏光 ,
  • 李建鸿 ,
  • 黄盛 ,
  • 柳维林 ,
  • 陶静 ,
  • 陈立典
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  • 1.福建中医药大学康复医学院,福建福州市 350122
    2.福建省康复技术协同创新中心,福建福州市 350122
    3.康复医疗技术国家地方联合工程研究中心,福建福州市 350122
    4.国家中医药管理局中医康复研究中心,福建福州市 350122
黄佳(1984-),女,汉族,重庆市人,博士研究生,主要研究方向：神经康复及认知科学。

收稿日期: 2017-09-17

  修回日期: 2017-11-09

  网络出版日期: 2018-01-31

基金资助

1.国家自然科学基金项目(No.81403462); 2.福建省卫生厅青年科研项目(No.2014-1-70)

收起

Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats

  • HUANG Jia ,
  • SONG Chang-ming ,
  • YANG Min-guang ,
  • LI Jian-hong ,
  • HUANG Sheng ,
  • LIU Wei-lin ,
  • TAO Jing ,
  • CHEN Li-dian
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  • 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
    2. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China
    3. National & Local Joint Engineering Research Center of Rehabilitation Medical Technology, Fuzhou, Fujian 350122, China;
    4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received date: 2017-09-17

  Revised date: 2017-11-09

  Online published: 2018-01-31

Supported by

National Natural Science Foundation of China (No. 81403462) and Youth Research Project of Fujian Health Department (No. 2014-1-70)

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摘要

目的 观察电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆功能的影响,并探究其可能机制。方法 雄性Sprague-Dawley大鼠42只随机分为假手术组(n=12)和手术组(n=30)。手术组线栓法制备左侧大脑中动脉栓塞90 min再灌注模型,符合纳入标准的24只分为模型组(n=12)和电针组(n=12)。电针组电针百会、神庭共7 d。造模后2 h和干预后1 d、3 d、7 d,采用Longa神经行为学评分进行评定;干预后3 d起行Barnes迷宫实验检测,共5 d;干预7 d后,免疫荧光标记法检测缺血侧海马CA1区嘌呤受体P2X7的表达,酶联免疫吸附法检测海马CA1区白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)。结果 干预后7 d,与模型组相比,电针组Longa评分降低(P<0.05);与假手术组相比,模型组Barnes迷宫逃避潜伏期显著延长(P<0.001),进入错误洞口次数显著增多(P<0.001);与模型组相比,电针组逃避潜伏期显著缩短(P<0.001),进入错误洞口次数显著减少(P<0.001);与假手术组比较,模型组P2X7受体平均光密度,IL-1β、TNF-α水平均显著提高(P<0.001);与模型组相比,电针组P2X7受体表达,IL-1β、TNF-α水平减少(P<0.05)。结论 电针百会、神庭穴能有效改善缺血再灌注损伤大鼠的学习记忆能力,可能与抑制海马CA1区嘌呤受体P2X7表达,改善缺血再灌注损伤大鼠海马CA1区神经炎症反应有关。

关键词： 脑缺血再灌注; 电针; 嘌呤受体; P2X7; 海马; 学习记忆; 炎症; 大鼠

本文引用格式

黄佳 , 宋长明 , 杨敏光 , 李建鸿 , 黄盛 , 柳维林 , 陶静 , 陈立典 . 电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响[J]. 中国康复理论与实践, 2018 , 24(1) : 43 -48 . DOI: 10.3969/j.issn.1006-9771.2018.01.008

Abstract

Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memory in rats after cerebral ischmeia-reperfusion and the possible mechanism.Methods A total of 42 male Sprague-Dawley rats were randomly divided into sham group (n=12) and operation group (n=30). The left middle cerebral arteries of the operation group were occluded with the modified Longa's method for 90 minutes and reperfused, and 24 qualified rats were randomly divided into model group (n=12) and electroacupuncture group (n=12), and the latter accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's score two hours after modeling, and one, three, seven days after intervention. They were tested with Barnes maze since three days after intervention, once a day for five days. The expression of purinoceptor P2X7 in CA1 of the hippocampus were detected with immunofluorescence seven days after intervention, while the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in CA1 were detected with enzyme-linked immunosorbent assay.Results The Longa's score was improved in the electroacupuncture group compared with that in the model group seven days after intervention (P<0.05); while the escape latency and the times entering the wrong hole increased in the model group compared with that in the sham group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.001). The expression of P2X7, IL-1β and TNF-α increased in the model group compared with the sham operation group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.05).Conclusion Electroacupuncture at Baihui and Shenting can improve the learning and memory in rats after cerebral ischemia-reperfusion, which may associate with inhibition of P2X7 to alleviate inflammation in hippocampus.

Key words： cerebral ischemia-reperfusion; electroacupuncture; purinoceptor; P2X7; hippocampus; learning and memory; inflammation; rats

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