双光子显微镜在小动物活体光学成像中的研究进展

doi:10.3969/j.issn.1006-9771.2017.01.009

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (1): 37-41.doi: 10.3969/j.issn.1006-9771.2017.01.009

双光子显微镜在小动物活体光学成像中的研究进展

张文豪^{1, 3, 4}, 李建军^{1, 3, 4}, 杨德刚^{1, 3, 4}, 杨明亮^{1, 3, 4}, 杜良杰^{1, 3, 4}, 高峰^{1, 3, 4}, 刘长彬^{1, 3, 4}, 李大鹏^{1, 3, 4}, 胡安明¹, 蔡畅^{1, 3, 4}

1.首都医科大学康复医学院,北京市 100068;
2.中国康复研究中心北京博爱医院,a.脊柱脊髓神经功能重建科;
b.神经外科,北京市 100068;
3.北京脑重大疾病研究院神经损伤与修复研究所,北京市 100068;
4.北京市神经损伤与康复重点实验室,北京市 100068。

收稿日期:2016-10-19 出版日期:2017-01-20 发布日期:2017-02-17
作者简介:张文豪(1991-),男,汉族,河南柘城县人,硕士研究生,主要研究方向：脊柱脊髓损伤的康复与治疗。通讯作者：李建军(1962-),男,汉族,教授,主任医师,博士研究生导师,主要研究方向：骨科及脊柱脊髓损伤的康复与治疗。E-mail: crrc100@163.com。
基金资助:
国家自然科学基金项目(No.81272164); 中央级公益性科研院所基本科研业务费专项资金(No.2015CZ-6); 中国康复研究中心课题(No.2012-No.2013-7)

Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)

ZHANG Wen-hao^{1, 3, 4}, LI Jian-jun^{1, 3, 4}, YANG De-gang^{1, 3, 4}, YANG Ming-liang^{1, 3, 4}, DU Liang-jie^{1, 3, 4}, GAO Feng^{1, 3, 4}, LIU Chang-bin^{1, 3, 4}, LI Da-peng^{1, 3, 4}, HU An-ming¹, CAI Chang^{1, 3, 4}

1. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China;
2. a. Department of Spinal and Neural Function Reconstruction; b. Department of Neurosurgery, China Rehabilitation Research Center, Beijing Bo'ai Hospital, Beijing 100068, China;
3. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100068, China;
4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, China

Received:2016-10-19 Published:2017-01-20 Online:2017-02-17
Contact: LI Jian-jun. E-mail: crrc100@163.com

摘要/Abstract

摘要： 双光子显微镜结合了激光扫描共聚焦显微镜和双光子激发技术。双光子荧光显微镜具有光损伤小、漂白区域小、穿透能力强、高分辨率、荧光收集率高、图像对比度高、可实现暗场成像、适合多标记复合测量等多种特点,可应用于小动物活体光学成像,在肿瘤免疫治疗、基因治疗、干细胞研究、药物筛选与评价、活体脊髓损伤成像等领域研究中有广泛应用。

关键词: 双光子显微镜, 活体光学成像, 小动物, 综述

Abstract: Twophoton microscopy is a new technique which combines laser scanning con-focal microscopy and two-photon excitation technique. Two-photon fluorescence microscopy has the advantages of little light damage, small bleaching area, strong penetrability, high resolution, high fluorescence collection efficiency, and high image contrast. It is suitable for dark field imaging and multi-labeled compound measurement, and has been widely used in small animals in vivo optical imaging, such as research for tumour, gene therapy, stem cells, drug development, spinal cord injury, etc.

Key words: two-photon microscopy, in vivo optical imaging, small animals, review

中图分类号:

R446.8

张文豪, 李建军, 杨德刚, 杨明亮, 杜良杰, 高峰, 刘长彬, 李大鹏, 胡安明, 蔡畅. 双光子显微镜在小动物活体光学成像中的研究进展[J]. 《中国康复理论与实践》, 2017, 23(1): 37-41.

ZHANG Wen-hao, LI Jian-jun, YANG De-gang, YANG Ming-liang, DU Liang-jie, GAO Feng, LIU Chang-bin, LI Da-peng, HU An-ming, CAI Chang. Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(1): 37-41.

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双光子显微镜在小动物活体光学成像中的研究进展

Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)

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