有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制

doi:10.3969/j.issn.1006-9771.2017.06.009

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (6): 662-666.doi: 10.3969/j.issn.1006-9771.2017.06.009

有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制

张崇林, 刘绍生, 夏志, 王世香, 丁孝民, 王前进, 王卉

井冈山大学体育学院,江西吉安市 343009。

收稿日期:2016-09-27 修回日期:2016-10-28 出版日期:2017-06-25 发布日期:2017-06-27
通讯作者: 王卉(1978- ),女,内蒙乌海市人,讲师,博士,主要研究方向：运动人体科学。E-mail: jgswh@163.com。
作者简介:张崇林(1976-),男,汉族,湖北孝感市人,博士,副教授,主要研究方向：运动人体科学。
基金资助:
1.江西省教育厅科技计划项目(No.GJJ150772); 2.井冈山大学博士科研启动项目(自然科学) (No.JZB1314; No.JZB11042)

Effect of Aerobic Exercises and Dietary Intervention on Lipid Metabolism in Rats with Metabolic Syndrome and Mechanism Medicated by Peroxisome Proliferator-Activated Receptor α

ZHANG Chong-lin, LIU Shao-sheng, XIA Zhi, WANG Shi-xiang, DING Xiao-min, WANG Qian-jin, WANG Hui

Jinggangshan University, Ji'an, Jiangxi 343009, China

Received:2016-09-27 Revised:2016-10-28 Published:2017-06-25 Online:2017-06-27
Contact: Correspondence to WANG Hui. E-mail: jgswh@163.com

摘要/Abstract

摘要： 目的通过对代谢综合征大鼠施加运动和膳食干预,观察有氧运动对代谢综合征脂代谢紊乱的调节作用,并探讨过氧化物酶体增殖物激活受体α (PPARα)介导的可能机制。方法 Sprague-Dawley大鼠喂养1周后随机分为空白对照组(CC组)和造模组,后者高脂高盐饲料喂养18周。造模成功后,将成模大鼠随机分为模型对照组(MC组,n=9)、模型高脂运动组(MHE组,n=11)和模型普食运动组(ME组,n=11)。ME组和MHE组跑台训练12周后测定体质量、肾周脂质量、血游离脂肪酸(FFA)、血脂,用荧光定量RT-PCR和Western blotting法测定心肌组织中的PPARα mRNA和蛋白表达水平。结果与CC组相比,MC组体质量、肾周脂质量、FFA、血脂升高(P<0.05),PPARα mRNA和蛋白表达水平均明显降低(P<0.01);与MC组比较,MHE组和ME组体质量、肾周脂质量、血三酰甘油(TG)较均降低(P<0.05),高密度脂蛋白(HDL)、PPARα mRNA和蛋白表达水平升高(P<0.05);与MHE组比较,ME组低密度脂蛋白(LDL)水平降低(P<0.05),PPARα mRNA和蛋白表达水平明显升高(P<0.01)。结论有氧训练能激活PPARα的表达,加强脂肪酸的利用,从而降低代谢综合征大鼠体质量和内脏脂肪质量,调节机体脂代谢。

关键词: 代谢综合征, 过氧化物酶体增殖物激活受体α, 有氧运动, 膳食干预, 脂代谢, 大鼠

Abstract: Objective To study the effect of aerobic exercise and dietary intervention on lipid metabolism in metabolic syndrome rats, and investigate the possible mechanism mediated by peroxisome proliferator-activated receptor α (PPAR α). Methods After one-week feeding, Sprague-Dawley rats were randomly divided into blank control group (CC group) and model group which were feed in high-fat-and-salt diet for 18 weeks to establish a metabolic syndrome model. Then, the metabolic syndrome rats were randomly divided into model control group (MC), the model high-fat diet group (MHE) and the model general died exercise group (ME). ME and MHE groups were forced to run on a treadmill for twelve weeks at the same time. The weight of perirenal fat, blood free fat acid (FFA), and blood lipid were determined. The expression of PPARα mRNA in myocardium was detected by RT-PCR. Western blotting was applied to detect the protein expression of PPARα in myocardium. Results Compared with CC group, MC group showed significant increase in body weight, perirenal fat weigh, FFA, and blood lipid (P<0.05), and significant decrease in PPARα mRNA and protein expression (P<0.01) in myocardium. Compared with MC group, ME and MHE groups showed significant decrease in body weight, perirenal fat weight, triglyceride (TG), and showed significant increase in high-density lipoprotein (HDL), and the expression of PPARα mRNA and protein in myocardium (P<0.05). Compared with MHE group, ME group showed decrease in low-density lipoprotein (LDL) (P<0.05), and increase in the expression of PPARα mRNA and protein (P<0.01). Conclusion Aerobic exercise may activate the expression of PPARα, enhance the utilization of fatty acid, reduce body mass and visceral fat mass, improve the dyslipidemia and then regulate lipid metabolism in metabolic syndrome rats.

Key words: metabolic syndrome, peroxisome proliferator-activated receptor α, aerobic exercise, dietary intervention, lipid metabolism, rats

中图分类号:

R589

张崇林, 刘绍生, 夏志, 王世香, 丁孝民, 王前进, 王卉. 有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制[J]. 《中国康复理论与实践》, 2017, 23(6): 662-666.

ZHANG Chong-lin, LIU Shao-sheng, XIA Zhi, WANG Shi-xiang, DING Xiao-min, WANG Qian-jin, WANG Hui. Effect of Aerobic Exercises and Dietary Intervention on Lipid Metabolism in Rats with Metabolic Syndrome and Mechanism Medicated by Peroxisome Proliferator-Activated Receptor α[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(6): 662-666.

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有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制

Effect of Aerobic Exercises and Dietary Intervention on Lipid Metabolism in Rats with Metabolic Syndrome and Mechanism Medicated by Peroxisome Proliferator-Activated Receptor α

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