电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响

doi:10.3969/j.issn.1006-9771.2017.10.005

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (10): 1145-1151.doi: 10.3969/j.issn.1006-9771.2017.10.005

电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响

金昊¹, 柳维林¹, 林冰冰¹, 李珑¹, 彭洪卫¹, 张秀峰¹, 宋长明¹, 黄佳¹, 陶静^{1, 2, 3}, 陈立典^{1, 2, 3, 4}

1.福建中医药大学康复医学院,福建福州市 350122;
2.福建省康复技术协同创新中心,福建福州市 350122;
3.康复医疗技术国家地方联合工程研究中心,福建福州市 350122;
4.国家中医药管理局中医康复研究中心,福建福州市350122

收稿日期:2017-04-17 修回日期:2017-06-02 出版日期:2017-10-25 发布日期:2017-11-03
通讯作者: 陈立典(1963-),男,汉族,福建政和县人,博士,教授,主要研究方向：神经康复与认知科学研究。E-mail: cld@fjtcm.edu.cn。
作者简介:金昊(1989-),男,汉族,安徽阜阳市人,硕士研究生,主要研究方向：神经康复与认知科学研究。
基金资助:
国家自然科学基金项目(No.81403450)

Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expression of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion

JIN Hao¹, LIU Wei-lin¹, LIN Bing-bing¹, LI Long¹, PENG Hong-wei¹, ZHANG Xiu-feng¹, SONG Chang-ming¹, HUANG Jia¹, TAO Jing^{1, 2, 3}, CHEN Li-dian^{1, 2, 3, 4}

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
2. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China;
3. National & Local Joint Engineering Research Center of Rehabilitation Medical Technology, Fuzhou, Fujian 350122, China;
4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received:2017-04-17 Revised:2017-06-02 Published:2017-10-25 Online:2017-11-03
Contact: CHEN Li-dian. E-mail: cld@fjtcm.edu.cn

摘要/Abstract

摘要： 目的探讨电针百会、神庭穴对脑缺血再灌注(MCAO/R)大鼠学习记忆的影响及其可能的作用机制。方法将90只健康雄性Sprague-Dawley大鼠随机分为假手术组(n=18)和手术组(n=72)。手术组参照线栓法制备MCAO/R大鼠模型。将符合纳入标准的54只手术组大鼠随机分为模型组(n=18)、电针组(n=18)和非穴组(n=18)。电针组电针百会、神庭穴共14 d。小动物磁共振扫描检测脑梗死体积;Morris水迷宫测试检测学习记忆能力;免疫组化检测5-羟色胺1A(5-HT_1A)受体的定位和表达。结果干预前各组大鼠脑梗死体积无显著性差异(F=1.678, P>0.05)。干预后,与模型组和非穴组相比,电针组脑梗死体积显著减小(P<0.001)。Morris水迷宫测试中,电针组较模型组和非穴组逃避潜伏期显著缩短(P<0.001),穿越平台次数增加(P<0.05)。5-HT_1A受体在缺血侧海马CA1、CA3和DG区均出现表达,电针组表达量较模型组和非穴组均降低(P<0.05)。非穴组各项指标与模型组比较均无显著性差异(P>0.05)。结论电针百会、神庭穴可有效改善 MCAO/R大鼠的学习记忆能力,其机制可能与调节海马区5-HT_1A受体表达有关。

关键词: 脑缺血再灌注, 电针, 学习记忆, 5-羟色胺1A受体, 大鼠

Abstract: Objective To explore the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) acupoints on learning and memory ability in rats with middle cerebral artery occlusion and reperfusion (MCAO/R), and the potential mechanisms.Methods A total of 90 healthy male Sprague-Dawley rats were randomly divided into sham group (n=18) and operation group (n=72). The MCAO/R model was established by suture method in the operation group. Finally, 54 qualified rats of the operation group were randomly divided into ischemia group (n=18), electroacupuncture group (n=18) and non-acupoint group (n=18). The electroacupuncture group received electroacupuncture at Baihui (DU20) and Shenting (DU24) for 14 days. The cerebral infarction volume was measured by magnetic resonance imaging (MRI). The learning and memory ability was tested by Morris water maze. The protein expression of 5-hydroxytryptamine 1A (5-HT_1A) receptor was detected by immunohistochemistry.Results There was no significant difference in the cerebral infarction volume among three groups before intervention (F=1.678, P>0.05). Compared with the ischemia group and the non-acupoint group, the cerebral infarction volume significantly reduced (P<0.001); the latency significantly shortened (P<0.001) and the times crossing the platform decreased (P<0.05); the expression of 5-HT_1A receptor decreased in the left hippocampus (P<0.05) in the electroacupuncture group after intervention. There was no significant difference in all the indices between the non-acupoint group and the ischemia group after intervention (P>0.05).Conclusion Electroacupuncture at Baihui (DU20) and Shenting (DU24) could effectively increase the learning and memory ability of MCAO/R rats, which might relate with inhibiting the expression of 5-HT_1A receptor in hippocampus.

Key words: cerebral ischemia-reperfusion, electroacupuncture, learning and memory, 5-hydroxytryptamine 1A receptor, rats

中图分类号:

R743.3

金昊, 柳维林, 林冰冰, 李珑, 彭洪卫, 张秀峰, 宋长明, 黄佳, 陶静, 陈立典. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响[J]. 《中国康复理论与实践》, 2017, 23(10): 1145-1151.

JIN Hao, LIU Wei-lin, LIN Bing-bing, LI Long, PENG Hong-wei, ZHANG Xiu-feng, SONG Chang-ming, HUANG Jia, TAO Jing, CHEN Li-dian. Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expression of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(10): 1145-1151.

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电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响

Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expression of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion

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