沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响

doi:10.3969/j.issn.1006-9771.2018.01.012

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (1): 66-70.doi: 10.3969/j.issn.1006-9771.2018.01.012

沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响

胡析, 牛英杰, 黄媛, 李盈洁, 曾茜, 向阳, 张晓, 梁楠

1.成都医学院,a.临床医学院,b.检验医学院,c.基础医学院实验教学中心,四川成都市 610500

收稿日期:2017-07-20 修回日期:2017-08-29 出版日期:2018-01-25 发布日期:2018-01-31
通讯作者: 梁楠,女,硕士,副教授,主要研究方向：脊髓损伤与修复;E-mail:33722677@qq.com。
作者简介:胡析(1995-),女,汉族,四川眉山市人,本科生。
基金资助:
1.国家级大学生创新创业训练计划项目(No.201613705028); 2.四川省大学生创新创业训练计划项目(No.201613705059)

Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion

HU Xi, NIU Ying-jie, HUANG Yuan, LI Ying-jie, ZENG Xi, XIANG Yang, ZHANG Xiao, LIANG Nan

1. a. Clinical Medicine College b. Department of Inspection c. Experiment Technology Center, Preclinical Medicine of Chengdu Medical College, Chengdu, Sichuan 610500, China

Received:2017-07-20 Revised:2017-08-29 Published:2018-01-25 Online:2018-01-31
Contact: LIANG Nan.E-mail:33722677@qq.com
Supported by:
National Students' Innovation and Entrepreneurship Training Program (No. 201613705028) and Sichuan Province Students' Innovation and Entrepreneurship Training Program (No. 201613705059)

摘要/Abstract

摘要： 目的探讨白细胞介素-1β (IL-1β)对大鼠脊髓钝挫伤模型中波形蛋白的影响。方法 Sprague-Dawley大鼠30只,采用Allen法制备脊髓钝挫伤模型,随机分为空载组(n=15)和干扰组(n=15),分别注射空质粒和包装IL-1β siRNA的质粒;每组再分为3 d、7 d和28 d亚组。通过GeneMANIA预测IL-1β与波形蛋白之间的关系。采用免疫组化和实时定量聚合酶链反应方法观察损伤区波形蛋白的表达。结果 GeneMANIA分析显示,IL-1β与波形蛋白可通过多个基因直接或间接联系。空载组波形蛋白在脊髓前角神经元和神经胶质细胞中广泛表达,干扰组波形蛋白及mRNA均较空载组下降(t>2.875, P<0.05)。结论 IL-1β能调控波形蛋白表达,可能影响损伤修复。

关键词: 脊髓钝挫伤, 白细胞介素-1β, 波形蛋白, siRNA, 大鼠

Abstract: Objective To observe the expression of vimentin (Vim) after silence of interleukin-1β (IL-1β) in rats with spinal cord contusion (SCC).Methods The model of SCC was established in 30 Sprague-Dawley rats with Allen's method. The rats were randomized into vector group (n=15) and silence group (n=15), which were injected blank lentivirus vector and vector of IL-1β siRNA, respectively; and divided in three, seven and 28 days subgroups. The relationship between IL-1β and Vim was predicted with GeneMANIA bioinformatics. The expression of Vim protein and mRNA in spinal cord was detected with immunohistochemistry and real-time quantitative polymerase chain reaction.Results GeneMANIA bioinformatic analysis indicated that there was some direct and indirect relationship between IL-1β and Vim. The Vim protein and mRNA expressed in the spinal cord, and was less in the silence group than in the vector group (t>2.875, P<0.05). Conclusion Silence of IL-1β can inhibit the expression of Vim in SCC rats, which may promote the recovery of spinal cord function.

Key words: spinal cord contusion, interleukin-1β, vimentin, siRNA, rats

中图分类号:

R651.2

胡析, 牛英杰, 黄媛, 李盈洁, 曾茜, 向阳, 张晓, 梁楠. 沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响[J]. 《中国康复理论与实践》, 2018, 24(1): 66-70.

HU Xi, NIU Ying-jie, HUANG Yuan, LI Ying-jie, ZENG Xi, XIANG Yang, ZHANG Xiao, LIANG Nan. Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 66-70.

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沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响

Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion

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