推拿联合跑轮训练对大鼠失神经骨骼肌萎缩的效果

doi:10.3969/j.issn.1006-9771.2018.05.008

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (5): 530-534.doi: 10.3969/j.issn.1006-9771.2018.05.008

推拿联合跑轮训练对大鼠失神经骨骼肌萎缩的效果

马颖¹, 严隽陶¹, 马书杰², 陆永嘉², 王春红¹, 史智君¹, 陶然², 孔亚敏¹

1.上海中医药大学附属岳阳中西医结合医院推拿科,上海市 200437;
2. 上海中医药大学,上海市 201203

收稿日期:2018-01-08 修回日期:2018-04-04 出版日期:2018-05-25 发布日期:2018-05-24
通讯作者: 严隽陶。E-mail: doctoryjt@sohu.com
作者简介:马颖(1991-),女,回族,甘肃天水市人,硕士研究生,主要研究方向：传统康复促进周围神经再生及防治失神经骨骼肌萎缩的基础研究与临床应用。
基金资助:
1.国家自然科学基金项目(No. 30973806); 2.国家自然基金青年科学基金项目(No. 81603713); 3.上海市中医药发展三年行动计划项目(No. ZY3-LCPT-1-1008)

Effect of Tuina and Treadmill Training on Skeletal Muscle Atrophy after Denervation in Rats

MA Ying¹, YAN Jun-tao¹, MA Shu-jie², LU Yong-jia², WANG Chun-hong¹, SHI Zhi-jun¹, TAO Ran², KONG Ya-min¹

1. Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditonal Chinese Medicine, Shanghai 200437, China;
2. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Received:2018-01-08 Revised:2018-04-04 Published:2018-05-25 Online:2018-05-24
Contact: YAN Jun-tao. E-mail: doctoryjt@sohu.com
Supported by:
Supported by National Natural Science Foundation of China (No. 30973806), National Natural Science Foundation of China (Youth) (No. 81603713) and Shanghai Three-year Plan for Traditional Chinese Medicine (No. ZY3- LCPT-1-1008)

摘要/Abstract

摘要： 目的探讨推拿手法联合跑轮训练对失神经骨骼肌萎缩的作用及其机制。方法 1月龄Sprague-Dawley大鼠80只,切断右下肢坐骨神经后随机分为对照组和手法组,每组40只。手法组予捏法推拿联合跑轮训练,对照组不予干预。分别在干预后1、2、3、4个月,每组各取10只大鼠检测腓肠肌湿重、肌卫星细胞计数,免疫组化检测胰岛素样生长因子Ⅰ(IGF-Ⅰ)阳性细胞计数,腓肠肌行HE染色观察。结果与对照组相比,手法组肌湿重比干预后3个月降低(F=4.590, P<0.05);肌卫星细胞计数在干预后3个月明显增加(F=12.466, P<0.01),IGF-Ⅰ阳性细胞数在干预后2个月后均增加(F>6.489, P<0.05)。HE染色示,手法组损伤程度有所减轻。结论推拿手法联合跑轮训练能够在一定程度上减轻失神经骨骼肌损伤,但不足以减轻肌萎缩,这可能是通过促进肌卫星细胞和IGF-Ⅰ的表达实现的。

关键词: 骨骼肌萎缩, 失神经, 推拿, 运动训练, 肌卫星细胞, 胰岛素样生长因子, 大鼠

Abstract: Objective To explore the effect of the combination of Tuina and treadmill training on denervation skeletal muscle atrophy. Methods A total of 80 Sprague-Dawley rats (one month old) were randomly divided into control group (n=40) and manipulation group (n=40). Their sciatic nerves were transected, and the manipulative group accepted treadmill training and kneading of Tuina, while the control group accepted no intervention. Their muscle wet weight ratio, muscle satellite cells and insulin-like growth factor I (IGF-I) positive cells count were measured, and HE staining of gastrocnemius muscle were observed one, two, three and four months after intervention, ten rats in each group. Results Compared with the control group, the muscle wet weight ratio decreased three months after intervention (F=4.590, P<0.05), muscle satellite cells increased three months after intervention (F=12.466, P<0.01), and IGF-I positive cells increased two, three and four months after intervention (F>6.489, P<0.05). HE staining showed the skeletal muscle injury relieved somehow.Conclusion The combination of Tuina and treadmill training can relieve denervation skeletal muscle injury, but it is not enough for skeletal muscle atrophy, which may associate with promoting the expression of muscle satellite cells and IGF-I.

Key words: skeletal muscle atrophy, denervation, Tuina, exercise, satellite cells, insulin-like growth factor, rats

中图分类号:

R746.4

马颖, 严隽陶, 马书杰, 陆永嘉, 王春红, 史智君, 陶然, 孔亚敏. 推拿联合跑轮训练对大鼠失神经骨骼肌萎缩的效果[J]. 《中国康复理论与实践》, 2018, 24(5): 530-534.

MA Ying, YAN Jun-tao, MA Shu-jie, LU Yong-jia, WANG Chun-hong, SHI Zhi-jun, TAO Ran, KONG Ya-min. Effect of Tuina and Treadmill Training on Skeletal Muscle Atrophy after Denervation in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 530-534.

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推拿联合跑轮训练对大鼠失神经骨骼肌萎缩的效果

Effect of Tuina and Treadmill Training on Skeletal Muscle Atrophy after Denervation in Rats

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