早期运动联合戊四氮对脑缺血再灌注大鼠神经功能的效果及其机制

doi:10.3969/j.issn.1006-9771.2021.01.003

《中国康复理论与实践》 ›› 2021, Vol. 27 ›› Issue (1): 27-30.doi: 10.3969/j.issn.1006-9771.2021.01.003

早期运动联合戊四氮对脑缺血再灌注大鼠神经功能的效果及其机制

彭志锋, 张义平, 张继红

山西大同大学医学院生理系教研室，山西大同市 037009

收稿日期:2020-03-13 修回日期:2020-04-03 出版日期:2021-01-25 发布日期:2021-01-27
通讯作者: 彭志锋，E-mail： pzf181@126.com
作者简介:彭志锋(1979-)，男，汉族，山西大同市人，博士，副教授，主要研究方向：缺血性脑卒中干预措施及分子机制。
基金资助:
山西大同大学博士科研启动经费项目(No. 2014-B-01)

Effect of Early Exercise Combined with Pentylenetetrazol on Neurologic Function after Cerebral Ischemia-reperfusion in Rats and Its Mechanism

PENG Zhi-feng, ZHANG Yi-ping, ZHANG Ji-hong

Department of Physiology, Medical College of Shanxi Datong University, Datong, Shanxi 037009, China

Received:2020-03-13 Revised:2020-04-03 Published:2021-01-25 Online:2021-01-27
Contact: PENG Zhi-feng, E-mail: pzf181@126.com
Supported by:
Shanxi Datong University Doctoral Research Start-Up Fund (No. 2014-B-01)

摘要/Abstract

摘要： 目的探讨早期跑步运动和γ-氨基丁酸(GABA)受体拮抗剂对缺血再灌注(I/R)大鼠神经功能和脑源性神经营养因子(BDNF)表达的影响。方法雄性Sprague-Dawley大鼠50只平均分为假手术组、模型组、运动组、戊四氮组和综合组，每组10只。除假手术组外，其余大鼠行大脑中动脉阻塞(MCAO) 1 h再灌注。再灌注2 d后，戊四氮组和综合组每天腹腔注射戊四氮0.25 mg/kg，共5 d；运动组和综合组每天跑步机上跑步30 min。再灌注7 d后，各组大鼠行神经行为学评价，TTC染色评估各组大鼠梗死体积，逆转录聚合酶链反应(RT-PCR)和ELISA法检测各组缺血半影区BDNF表达水平。结果与模型组相比，运动组、戊四氮组和综合组神经功能评分改善，脑梗死体积减少(P < 0.05)，而且综合组优于其他两组(P < 0.05)。综合组缺血半影区BNDF表达高于其他组(P < 0.05)。结论早期跑步运动联合戊四氮可促进I/R大鼠神经功能恢复，可能与BDNF上调有关。

关键词: 脑缺血/再灌注, 早期运动, γ-氨基丁酸受体拮抗剂, 大鼠

Abstract: Objective To observe the interactive effects of early exercise and γ-aminobutyric acid (GABA) receptor antagonists on the neurologic function and brain-derived neurotrophic factor (BDNF) expression in the cerebral ischemia-reperfusion (I/R) rat model. Methods Male Sprague-Dawley rats were divided into sham (n = 10), I/R (n = 10), exercise (EX) (n = 10), pentetrazol (PTZ) (n = 10) and pentetrazol plus exercise (PTZEX) (n = 10) groups. All the rats, except the sham group, accepted middle cerebral artery occlusion (MCAO) for one hour and reperfused. Since two days after reperfusion, PTZ and PTZEX groups accepted PTZ, a GABA receptor antagonist, 0.25 mg/kg peritoneal injection, once a day for five days; while EX and PTZEX groups ran on a treadmill, 30 minutes a day for five days. Seven days after reperfusion, all the rats were assessed with neurobehavioral score, the infarct volumes were assessed with TTC, and BDNF expression in ischemic penumbra was detected with reverse transcription polymerase chain reaction and ELISA. Results Compared with I/R group, the neurobehavioral scores of EX group, PTZ group and PTZEX group improved, the volumes of cerebral infarction reduced (P < 0.05), and PTZEX group was the best (P < 0.05). The expression of BNDF was the most in PTZEX group (P < 0.05). Conclusion Early exerxise combined with PTZ could promote the recovery of neurologic function in I/R rats, which may be related to the up-regulation of BDNF.

Key words: cerebral ischemia-reperfusion, early exercise, γ-aminobutyric acid receptor antagonists, rats

中图分类号:

R743.32

彭志锋, 张义平, 张继红. 早期运动联合戊四氮对脑缺血再灌注大鼠神经功能的效果及其机制[J]. 《中国康复理论与实践》, 2021, 27(1): 27-30.

PENG Zhi-feng, ZHANG Yi-ping, ZHANG Ji-hong. Effect of Early Exercise Combined with Pentylenetetrazol on Neurologic Function after Cerebral Ischemia-reperfusion in Rats and Its Mechanism[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(1): 27-30.

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早期运动联合戊四氮对脑缺血再灌注大鼠神经功能的效果及其机制

Effect of Early Exercise Combined with Pentylenetetrazol on Neurologic Function after Cerebral Ischemia-reperfusion in Rats and Its Mechanism

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