电针对血管性认知障碍大鼠脑功能局部一致性的效果

doi:10.3969/j.issn.1006-9771.2022.01.009

《中国康复理论与实践》 ›› 2022, Vol. 28 ›› Issue (1): 55-61.doi: 10.3969/j.issn.1006-9771.2022.01.009

电针对血管性认知障碍大鼠脑功能局部一致性的效果

丁妍怡¹,张胜行²,刘雨露¹,余燕¹,杨敏光²,梁胜祥²,柳维林²(),陶静¹

1.福建中医药大学康复医学院,福建福州市 350122
2.福建中医药大学康复产业研究院,福建福州市 350122

收稿日期:2021-09-03 修回日期:2021-11-15 出版日期:2022-01-25 发布日期:2022-02-11
通讯作者: 柳维林 E-mail:liuweilin12@fjtcm.edu.cn
作者简介:丁妍怡(1995-),女,汉族,安徽亳州市人,硕士研究生,主要研究方向：神经康复与认知科学研究。
基金资助:
国家自然科学基金项目(81873355)

Effect of electroacupuncture on regional homogeneity of brain function in rats with vascular cognitive impairment

DING Yanyi¹,ZHANG Shenghang²,LIU Yulu¹,YU Yan¹,YANG Minguang²,LIANG Shengxiang²,LIU Weilin²(),TAO Jing¹

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2. The Academy of Rehabilitation Industry, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received:2021-09-03 Revised:2021-11-15 Published:2022-01-25 Online:2022-02-11
Contact: LIU Weilin E-mail:liuweilin12@fjtcm.edu.cn
Supported by:
National Natural Science Foundation of China(81873355)

摘要/Abstract

摘要：

目的观察电针百会、神庭对血管性认知障碍(VCI)大鼠脑区功能活动和工作记忆的影响。方法 18只Sprague-Dawley大鼠,其中12只结扎双侧颈总动脉造模,假手术组6只不结扎。造模大鼠随机分为模型组(n = 6)和电针组(n = 6),电针组电针百会和神庭,共4周。干预前后采用Y迷宫、Morris水迷宫进行评定;干预后行静息态功能磁共振成像扫描,计算局部一致性(ReHo)。结果干预前,与假手术组相比,模型组和电针组Y迷宫交替率显著降低(P < 0.001),Morris水迷宫逃避潜伏期升高(P < 0.05);干预后,电针组Y迷宫交替率较模型组增高(P < 0.05),Morris水迷宫工作记忆逃避潜伏期降低(P < 0.05)。与假手术组相比,模型组双侧海马、嗅觉皮质、感觉皮质、听觉皮质,左侧纹状体ReHo降低;与模型组相比,电针组双侧前额叶、海马、嗅觉皮质,左侧杏仁核ReHo升高。结论电针百会、神庭可改善VCI大鼠工作记忆,可能与调节前额叶、海马、杏仁核等脑区功能活动有关。

关键词: 血管性认知障碍, 电针, 工作记忆, 局部一致性

Abstract:

Objective To observe the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) on brain functional activity and working memory of rats with vascular cognitive impairment (VCI). Methods Eighteen Sprague-Dawley rats were included, in which twelve rats were ligated bilateral common carotid arteries and six rats were not ligated (sham group). The modeled rats were randomly divided into model group (n = 6) and electroacupuncture group (n = 6). The electroacupuncture group received electroacupuncture at Baihui and Shenting for four weeks. They were assessed with Y maze and Morris water maze before and after intervention, and scaned with resting-state functional magnetic resonance imaging after intervention to calculate regional homogeneity (ReHo). Results Compared with the sham group, alternation rate of Y maze decreased (P < 0.001), and escape latency of Morris water maze increased (P < 0.05) in the model group and the electroacupuncture group before intervention. Compared with the model group, alternation rate of Y maze increased (P < 0.05), and escape latency of Morris water maze decreased (P < 0.05) after intervention in the electroacupuncture group. Compared with the sham group, ReHo of bilateral hippocampus, olfactory cortex, sensory cortex and auditory cortex, and left striatum decreased in the model group; compared with the model group, ReHo of bilateral prefrontal lobe, hippocampus and olfactory cortex, and left amygdala increased in the electroacupuncture group. Conclusion Electroacupuncture at Baihui and Shenting can improve the memory function of VCI rats, which may be related to the functional activities of prefrontal lobes, hippocampus and amygdala.

Key words: vascular cognitive impairment, electroacupuncture, working memory, regional homogeneity

中图分类号:

R743.3

丁妍怡,张胜行,刘雨露,余燕,杨敏光,梁胜祥,柳维林,陶静. 电针对血管性认知障碍大鼠脑功能局部一致性的效果[J]. 《中国康复理论与实践》, 2022, 28(1): 55-61.

DING Yanyi,ZHANG Shenghang,LIU Yulu,YU Yan,YANG Minguang,LIANG Shengxiang,LIU Weilin,TAO Jing. Effect of electroacupuncture on regional homogeneity of brain function in rats with vascular cognitive impairment[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(1): 55-61.

图/表 7

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参考文献 46

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组别	n	干预前	干预后
假手术组	6	78.03±3.90	78.37±8.83
模型组	6	59.83±4.17^a	58.77±5.44^a
电针组	6	63.56±6.78^a	68.31±5.61^b,c
F值		21.181	12.429
P值		< 0.001	0.001

组别	n	第一象限	第二象限	第三象限	第四象限
假手术组	6	27.89±18.49	18.07±10.67	21.87±14.84	18.56±12.40
模型组	6	51.17±8.35^a	40.21±18.02^a	42.85±14.7^a	41.72±19.19^a
电针组	6	47.25±18.87^a	40.12±18.06^a	43.67±14.75^a	49.38±13.79^a
F值		4.213	3.830	4.202	6.510
P值		0.035	0.045	0.036	0.009

组别	n	第一象限	第二象限	第三象限	第四象限
假手术组	6	17.04±11.85	15.96±8.85	13.69±10.43	15.24±9.48
模型组	6	49.34±9.59^a	45.65±14.65^a	43.30±12.21^a	41.76±12.29^a
电针组	6	24.86±13.51^b	26.63±15.65^b	27.66±8.27^a,b	24.90±16.14^b
F值		12.311	7.569	12.107	6.481
P值		0.001	0.005	<0.001	0.009

组别	n	干预前	干预后
假手术组	6	48.03±11.12	40.32±17.52
模型组	6	47.28±12.35	44.45±16.46
电针组	6	50.62±9.64	41.81±16.26
F值		0.149	0.094
P值		0.862	0.911

脑区	模型组 vs. 假手术组		电针组 vs. 模型组
脑区	体素	t值	体素	t值
左前额叶			1	3.878
右前额叶			7	5.288
左侧海马	1	3.750	6	4.370
右侧海马	23	5.359	72	6.232
左侧嗅觉皮质	3	4.059	13	4.534
右侧嗅觉皮质	4	3.928	7	4.062
左侧感觉皮质	64	5.186	-	-
右侧感觉皮质	26	4.759	-	-
左侧纹状体	17	4.704	1	3.846
左侧听觉皮质	14	4.458	-	-
右侧听觉皮质	13	4.982	8	4.817
右侧背侧丘脑外侧核群	2	4.109	-	-
左侧杏仁核	-	-	18	4.840
左侧中脑下丘	-	-	5	4.644
左侧中脑上丘	-	-	6	4.438
右侧中脑被盖	-	-	7	4.472
右侧梨状皮质	-	-	6	5.472

电针对血管性认知障碍大鼠脑功能局部一致性的效果

Effect of electroacupuncture on regional homogeneity of brain function in rats with vascular cognitive impairment

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