《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (11): 649-651.

• 专题 • 上一篇    下一篇

细胞低温长期生存系统的研究以及在空间搭载中的应用

房青; 徐梅; 徐波; 李红艳; 向青; 陈志华; 刘轩; 唐劲天   

  1. 中日友好医院临床医学研究所生化-分子生物学研究室 北京市 100029
  • 收稿日期:2004-10-13 出版日期:2004-11-25 发布日期:2004-11-25

Development of long-term cell culture facility in hypothermia and its application in space biology research

FANG Qing,XU Mei,XU Bo, et al   

  1. Department of Biochemistry and Molecule Biology,Institute of Clinical Medical Science,China-Japan Friendship Hospital,Beijing 100029,China
  • Received:2004-10-13 Published:2004-11-25 Online:2004-11-25

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被引次数

9

摘要： 目的研制哺乳动物细胞低温长期生存系统并用于空间搭载,为空间辐射的诱变效应研究提供基础条件。方法将基因工程细胞、肿瘤细胞和干细胞培养于细胞低温长期生存系统中,搭载神舟4号飞船和第18颗返回式卫星,观察细胞存活情况。结果多种细胞可在此系统中存活多达26d,其中空间飞行18d。细胞返地后经短暂复温即可进行消化,可生长增殖形成单克隆。结论此简易的细胞培养系统可使细胞于空间搭载条件下长期存活,有利于研究空间辐射对增殖期哺乳动物细胞的诱变效应。

关键词: 低温, 细胞培养, 空间, 培养系统

Abstract: ObjectiveTo develop the facility of mammalian cell culture in hypothermia for long-term, and to lay the base for carrying mammalian cells to outer space and establishing outer space biomedical research stage. MethodsMultiple cell lines were maintained in the spacial designed hypothermia cell culture facility and carried to space by Shenzhou 4 and No.18 recoverable satellite. Cell morphology, cell multiplication and the ability to be monocloned were observed.ResultsCells were successfully maintained in the hypothermia cell culture facility for up to 26 days without obvious changes of cell morphology. The cells could normally grow, multiple and be monocloned after inoculated in 37 ℃ for 8 h.ConclusionA hypothermia cell culture facility was successfully established,which ensure technically mammalian cells to be carried by space craft and further research on space radiation.

Key words: hypothermia, cell culture, spaceflight, culture facility