《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (05): 345-346.

• 基础研究 • 上一篇    下一篇

小鼠实验性自身免疫性脑脊髓炎性别差异研究

邢广羽; 张金涛; 宋春杰; 尹岭   

  1. 解放军总医院神经信息中心 北京市 100853
  • 收稿日期:2004-12-10 出版日期:2005-05-25 发布日期:2005-05-25

Study of the sex differences in experimental autoimmune encephalomyelitis

XING Guang-yu, ZHANG Jin-tao, SONG Chun-jie, et al   

  1. Department of Neuroinformation, The General Hospital of PLA, Beijing 100853, China
  • Received:2004-12-10 Published:2005-05-25 Online:2005-05-25

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被引次数

11

摘要： 目的探讨小鼠实验性自身免疫性脑脊髓炎(EAE)性别差异的原因。方法应用髓鞘脂质蛋白139-151(PLP139-151)免疫诱导建立EAE模型,观察雌雄小鼠病情的差异,采用MTT、ELISA法,检测外周淋巴细胞对自身抗原反应的性别差异。结果雌性小鼠的平均发病时间(15±2.1)d,早于雄性的(22±4.3)d,病程有复发-缓解的特点,而雄性则多呈一过性发病;EAE雌雄小鼠的特异性淋巴细胞增殖反应和γ-干扰素分泌无显著性差异(P>0.05),雄性小鼠白细胞介素4(IL-4)分泌明显高于雌性小鼠(P<0.01)。结论PLP139-151诱发小鼠EAE性别差异可能与雄性小鼠外周淋巴细胞分泌IL-4水平较高,具有保护作用有关。

关键词: 实验性自身免疫性脑脊髓炎(EAE), SJL/J小鼠, 性别差异, T淋巴细胞增殖, γ-干扰素, 白细胞介素-4(IL4-)

Abstract: ObjectiveTo explore the mechanism causing the sex differences in experimental autoimmune encephalomyelitis (EAE).MethodsEAE model was established with PLP 139-151 in SJL/J mice. The sex differences of illness state were evaluated by neurologic score, and that of response of peripheral lymphocytes to autologous antigens were detected by methods of MTT and ELISA.ResultsThe onset time of female SJL mice was 15±2.1 days less than that of male ones (22±4.3 days), and the feature of diseases course of female mice was relapse after recovery, but that of male mice was transient. No significant differences were observed in T cell responses and interferon-γ productions between male and female mice (P>0.05). Male mice secreted more interleukin-4 than female mice (P<0.01).ConclusionThe sex differences of EAE in mice are due to peripheral lymphocytes secreting more interleukin-4 in male mice.

Key words: experimental autoimmune encephalomyelitis (EAE), SJL/J mice, sex difference, T cell proliferative response, interferon-γ(IFN-γ), interleukin-4 (IL-4)