《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (01): 39-40.

• 基础研究 • 上一篇    下一篇

碱性成纤维细胞生长因子对体外培养新生牛视网膜色素上皮细胞生长周期的影响

张鲲; 何守志   

  1. 解放军总医院眼科 北京市 100853
  • 收稿日期:2005-10-08 出版日期:2006-01-25 发布日期:2006-01-25

Effects of basic fibroblast growth factor on growth phase of cultured bovine retinal pigment epithelium cells in vitro

ZHANG Kun,HE Shou-zhi   

  1. Department of Ophthalmology, General Hospital of PLA, Beijing 100853, China
  • Received:2005-10-08 Published:2006-01-25 Online:2006-01-25

PDF (PC)

摘要： 目的观察碱性成纤维细胞生长因子(bFGF)对体外培养新生牛视网膜色素上皮(RPE)细胞生长周期的影响。方法采用流式细胞仪测定bFGF4ng/ml作用下,不同时间RPE细胞的细胞周期情况。结果bFGF组与对照组比较,两组细胞各周期比例随时间变化趋势相同且同步,但bFGF组的变化幅度均大于对照组。结论bFGF可以促进新生牛RPE细胞自G1期进入S期,从而促进细胞增殖。

关键词: 碱性成纤维细胞生长因子(bFGF), 视网膜色素上皮细胞, 细胞周期, 增殖

Abstract: ObjectiveTo observe the effects of basic fibroblast growth factor (bFGF) on growth period of cultured bovine retinal pigment epithelium cells in different durations.MethodsDifferent proportion of phase with 4 ng/ml bFGF were measured by Flow Cytometry (FCM) assay.ResultsbFGF increased the RPE cells' proportion of phase S and decreased the proportion of phase G0-G1. ConclusionbFGF can stimulate RPE cells from phase G1 to phase S.

Key words: basic fibroblast growth factor (bFGF), retinal pigment epithelium cell, cell growth phase, proliferation