《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (01): 32-34.

• 专题　药物康复 • 上一篇    下一篇

不同剂量BPI-1095对大鼠局灶性脑缺血损伤的保护研究

王春雪1; 毛淑静2; 万虹3; 历俊华3; 翟晶1; 刘丽萍1; 王拥军1   

  1. 1.首都医科大学附属北京天坛医院神经内科,北京市 100050;2.北京护士学校药理组,北京市 101149;3.北京市神经外科研究所,北京市 100050
  • 收稿日期:2006-10-10 出版日期:2007-01-01 发布日期:2007-01-01

Neuroprotective Effects of different doses of BPI-1095 in Focal Cerebral Ischemia Rats

WANG Chun-xue, MAO Shu-jing , WAN Hong, et al   

  1. Department of Neurology, Beijing Tiantan Hospital Affiliated to the Capital Medical University, Beijing 100050, China
  • Received:2006-10-10 Published:2007-01-01 Online:2007-01-01

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摘要： 目的探讨BPI-1095对大鼠局灶性脑缺血损伤的保护作用。方法90只SD大鼠被随机分成6组:BPI-1095大剂量组(240mg/kg)、BPI-1095中剂量组(80mg/kg)、BPI-1095小剂量组(27mg/kg)、阿司匹林组(80mg/kg)、安慰剂组、假手术组,每组15只。于线栓法复制永久性大脑中动脉闭塞缺血模型术后10min,动物分别以灌胃的方式给予不同剂量的观察药物及对照药物。于给药后4h、24h采用动物行为学量表对缺血动物的神经功能进行评价。24h评分完成后,在造模成功且存活的各组动物中,每组随机取7或8只(使每组各剩余5只)用氯化三苯四唑(TTC)染色,利用计算机图像分析系统测定大脑半球梗死面积百分数。结果大剂量及中剂量BPI-1095可以改善缺血大鼠神经功能障碍(P<0.05);大、中剂量治疗组梗死面积百分数小于安慰剂组(P<0.05)。结论80～240mg/kgBPI-1095可以改善局灶性脑缺血大鼠的神经功能,缩小梗死体积。

关键词: 大脑中动脉闭塞(MCAO), 局灶性脑缺血, 动物行为学, 大鼠

Abstract: ObjectiveTo investigate the neuroprotective effects of different doses of BPI-1095 on infarct volume and neurological outcome in middle cerebral artery occlusion(MCAO) rats.MethodsCerebral ischemia model was induced with MCAO in adult male SD rats. 10 minutes after surgery, rats were randomly subjected into six groups with 15 rats in each group. Each rat has been given different dosage tested medication and was sacrificed 24 h after treatment. Neurological functional behaviour tests were performed 4 h and 24 h after treatment. After the final behaviour test, 7 or 8 rats (remain 5 rats for brain tissue stain) were randomly picked up from each group. Their infarction volume was measured with image analysis system after 2% triphenyl-tetrazolium chloride (TTC) stain. ResultsHigh dose (240 mg/kg) and moderate dose (80 mg/kg) of BPI-1095 were able to improve the neurological deficit in MCAO rats (P<0.05, vs vehicle-treated group), as well as they decrease the infracted volume (P<0.05, vs the vehicle-treated group ) 24 h after ischemia.Conclusion80～240 mg/kg BPI-1095 is able to improve neurological deficit effectively and reduce infarct volume significantly.

Key words: middle cerebral artery occlusion (MCAO), focal cerebral ischemia, animal behavior test, rats