《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (11): 1029-1031.

• 基础研究 • 上一篇    下一篇

16Hz次声作用对大鼠海马神经干细胞增殖能力的影响

唐晨1; 李玲2; 袁华1; 陈景藻1; 张美霞1   

  1. 1.第四军医大学西京医院康复与理疗科,陕西西安市 710032;2.解放军总医院第一附属医院康复理疗科,北京市 100037
  • 收稿日期:2007-07-05 出版日期:2007-11-01 发布日期:2007-11-01

Effect of 16 Hz Infrasound on Proliferation of Neural Stem Cells in Rats' Hippocampus

TANG Chen, LI Ling, YUAN Hua, et al   

  1. Department of Physiotherapy and Rehabilitation, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032,Shaanxi, China
  • Received:2007-07-05 Published:2007-11-01 Online:2007-11-01

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摘要： 目的研究16Hz次声作用对大鼠海马神经干细胞增殖能力的影响。方法将72只SD大鼠随机分为16Hz,130dB次声作用组(n=24)、16Hz,90dB次声作用组(n=24)以及对照组(n=24)。次声作用组大鼠暴露于16Hz,130dB和90dB的次声压力仓系统,2h/d,分别作用1d、7d、14d、21d。取脑进行巢蛋白(nestin)免疫组化染色,观察大鼠海马nestin标记的神经干细胞的变化情况。结果130dB组从作用1d开始,大鼠海马中nestin阳性细胞数量开始增加;随着作用次数增加而增多,于14d达到高峰,21d下降,但仍然高于对照组。90dB组大鼠各时间点nestin表达均比130dB组弱(P<0.05)。结论16Hz次声作用所致的脑损伤能刺激大鼠海马神经干细胞增殖,从而参与受损神经的修复过程。

关键词: 次声, 海马, 神经干细胞, 巢蛋白(nestin), 增殖

Abstract: Objective To study the effect of 16 Hz infrasound on the proliferation of the neural stem cells in rats' hippocampus.Methods72 SD rats were randomized into 16 Hz-130 dB infrasound exposure group(n=24),16 Hz-90 dB infrasound exposure group(n=24)and control group(n=24).Rats of the infrasound exposure groups were exposed to 16Hz 90dB/130dB infrasound barochamber system,2 h/d for 1 d,7 d,14 d,or 21 d.Their brains were removed and anti-nestin immunohistochemistry was used to observe the neural stem cells in rats' hippocampus.ResultsThe expression of nestin in rats' hippocampus increased after exposure for once and increased with the exposure time.It reached its peak on the 14th day,decreased on the 21st day but remained higher level.The reaction of 90 dB group was weaker than that of 130 dB group.ConclusionThe brain injuries result from 16 Hz infrasound exposure can enhance the proliferation of neural stem cells in rats' hippocampus which might participate in the repair of neural injuries.

Key words: infrasound, hippocampus, neural stem cells, nestin, proliferation