关键词: 缺血/再灌注损伤, 缺血后处理, 自由基, 药物后处理

Abstract: Objective To observe the effect of Edaravone pharmacological postconditioning and ischemic postconditioning on acute myocardial ischemia and reperfusion injury.Methods 40 rats were randomly divided into the sham operation group,ischemia/reperfusion group,ischemic postcondition group,Edaravone group A(injected through artery before reperfusion)and Edaravone group V(injected through vein before reperfusion)with 8 animals in each group.The animal model of acute myocardial ischemic and reperfusion was established.ECG changes were monitored during the procedure,dynamic parameters and serum biochemical markers creatine kinase MB(CK-MB),malondialdehyde(MDA),superoxide dismutase(SOD)of different groups were assessed and evaluated at the end of reperfusion.Ischemic and infarct areas were measured by Evans blue and TTC staining respectively.Results The myocardial infarct area and levels of serum CK-MB,MDA all significantly reduced(P<0.01)and activity of SOD enhanced(P<0.05)in the ischemic postcondition group and Edaravone group A compared with the ischemia/reperfusion group.The myocardial infarct area and levels of serum CK-MB,MDA reduced and activity of SOD enhanced(P<0.05)in the Edaravone group V compared with the ischemia/reperfusion group.There were no statistical differences in the foregoing indexes among the ischemic postcondition group,Edaravone group A and Edaravone group V group(P>0.05).Conclusion Edaravone injected through artery just before the onset of coronary reperfusion can reduce myocardial infarct area,which is similar to the cardio protective effect of mechanical postconditioning.The common potential mechanism of them might be associated with decreasing the injury by reactive oxygen species and strengthening the resistance to oxidation stress.The effect of intra aorta root-coronary injection is not worse than that of intra vein

Key words: ischemia/reperfusion injury, ischemic postconditioning, free radical, pharmacological postconditioning