《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (06): 531-532.

• 基础研究 • 上一篇    下一篇

枸杞多糖对2型糖尿病大鼠胰岛素抵抗及脂联素基因表达的影响

宗灿华; 田丽梅   

  1. 牡丹江医学院生物化学与分子生物学教研室,黑龙江牡丹江市 157011
  • 收稿日期:2008-03-31 出版日期:2008-06-01 发布日期:2008-06-01

Effect of Lycium Barbarum Polysaccharide on Insulin Resistance and Gene Expression of Adiponection in Type 2 Diabetes Mellitus Rats

ZONG Can-hua, TIAN Li-mei   

  1. The Department of Biochemistry and Molecular Biology, MudanjiangMedical College, Mudan- jiang 157011, Heilongjiang, China
  • Received:2008-03-31 Published:2008-06-01 Online:2008-06-01

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摘要： 目的观察枸杞多糖(LBP)对2型糖尿病(2-DM)大鼠胰岛素抵抗及肾周脂肪组织脂联素基因mRNA表达的影响。方法选用高脂饲料加小剂量链尿佐菌素建立2-DM大鼠模型,将2-DM大鼠随机分为模型对照组(水)和LBP高、中、低剂量组,每组10只,另选10只大鼠为正常对照组。各组灌胃8周后,RT-PCR法检测肾周白色脂肪组织脂联素基因mRNA水平,同时测定空腹血糖(FBG)、血清胰岛素(FINS)和血脂的含量,并计算胰岛素抵抗指数(IRI)。结果与模型组比较,LBP高、中剂量组2-DM大鼠肾周脂肪组织脂联素基因mRNA表达明显升高(P<0.01),LBP各剂量组FBG、FINS、IRI、总胆固醇、三酰甘油、低密度脂蛋白水平明显降低(P<0.05～0.01)。结论LBP可通过增加脂联素基因mRNA表达,改善2-DM大鼠脂代谢紊乱,降低胰岛素抵抗,从而实现其降血糖作用。

关键词: 枸杞多糖, 2型糖尿病, 胰岛素抵抗, 脂联素基因

Abstract: Objective To observe the effect of lycium barbarum polysaccharide (LBP) on insulin resistance and mRNA expression of adiponection gene in perirenal adipose tissue of type 2 diabetes mellitus (2-DM) rats.Methods The 2-DM model of rat was established by high-fat diet and streptozocin. The 2-DM model rats were randomly divided into the model group and high, middle and low doses of LBP groups with 10 animals in each group, another 10 rats were selected as the control group. The mRNA expression of adiponection gene in perirenal adipose tissue was determined by RT-PCR. The levels of fasting blood glucose (FBG), fasting insulin (FINS) and blood lipid total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected and insulin resistance index (IRI) was calculated after rats fed eight weeks.Results The mRNA expression of adiponection gene increased in the middle and high doses of LBP groups, while the levels of FBG, FINS, IRI, TC, TG and LDL decreased in different doses of LBP groups, significantly different from those in 2-DM model group.Conclusion LBP can improve the metabolism disorder of blood lipid and decrease insulin resistance by increasing mRNA expression of adiponection gene in 2-DM rats.

Key words: lycium barbarum polysaccharide, type 2 diabetes mellitus, insulin resistance, adiponection gene