《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (06): 535-536.

• 基础研究 • 上一篇    下一篇

DR5、DcR2在腰椎间盘中的表达及其意义

马斌; 牛涛; 胡有谷; 陈伯华   

  1. 青岛大学医学院附属医院脊柱外科,山东青岛市 266003
  • 收稿日期:2008-02-28 出版日期:2008-06-01 发布日期:2008-06-01

Expression of DR5 and DcR2 in Human Lumbar Intervertebral Disc

MA Bin, NIU Tao, HU You-gu, et al.   

  1. The Department of Orthopaedics, the Affiliated Hospital of Qingdao Medical College, Qingdao 266003, Shandong, China
  • Received:2008-02-28 Published:2008-06-01 Online:2008-06-01

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摘要： 目的观察DR5、DcR2在腰椎间盘组织中的表达分布及其意义。方法采用免疫组化技术检测腰椎间盘突出症60例、正常成人腰椎间盘22个(8例);实时荧光定量RT-PCR技术检测腰椎间盘突出症30例、正常成人椎间盘9个(3例)中DR5和DcR2的表达。结果免疫组化检测显示,腰椎间盘突出症患者DR5细胞阳性率为41.60%;正常腰椎间盘组细胞阳性率为26.09%,两者间的差异有非常高度显著性意义(P=0.001);DR5mRNA定量分析进一步支持免疫组化结果(P=0.025)。DcR2蛋白和mRNA表达两组间的差异无显著性意义。结论腰椎间盘组织中存在DR5/TRAIL诱导的凋亡途径。

关键词: 腰椎, 椎间盘, 肿瘤坏死因子相关凋亡诱导配体, 肿瘤坏死因子相关凋亡诱导配体受体

Abstract: Objective To investigate the expression of DR5 and DcR2 protein and mRNA in lumbar intervertebral disc (LID) of patients with prolapse of LID and normal controls.Methods The expression and distribution of DR5 and DcR2 protein were immunostained in prolapsed LIDs of 60 patients and 22 normal LIDs of 8 normal controls. In parallel, mRNA of DR5 and DcR2 was quantified by real time fluorescent reverse transcriptase-polymerase chain reaction (RT-PCR) in prolapsed LIDs of 30 patients and 9 normal LIDs of 3 normal controls.Results The positive rates of DR5 protein in prolapsed IVDs and normal IVDs were 41.60% and 26.09% respectively. There were a significant difference between the two groups ( P=0.001). Further similar evidences were obtained by quantification of DR5 mRNA ( P=0.025). There was no difference in the expression of DcR2 protein and mRNA between the two groups.Conclusion The apoptosis pathway induced by DR5/tumor necrotic factor-related apoptosis-inducing ligand (TRAIL) maybe exists in LID tissues.

Key words: lumbar vertebrae, intervertebral disc, tumor necrotic factor-related apoptosis-inducing ligand (TRAIL), TRAIL receptor