《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (01): 32-34.

• 基础研究 • 上一篇    下一篇

甾体皂苷TSA对局灶性脑缺血大鼠运动感觉功能的影响

李红艳1;孙建宁1;马百平2;从玉文2   

  1. 1.北京中医药大学中药学院中药药理系，北京市 100102;2军事医学科学院放射与辐射医学研究所，北京市 100850
  • 收稿日期:2008-09-02 出版日期:2009-01-01 发布日期:2009-01-01
  • 通讯作者: 孙建宁

Effect of Steroidal Saponin TSA on the Recovery of Sensorimotor Function after Cerebral Ischemia in Rats

LI Hong-yan, SUN Jian-ning, MA Bai-ping, et al   

  1. Beijing University of Chinese Medicine, Beijing 100102, China
  • Received:2008-09-02 Published:2009-01-01 Online:2009-01-01

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摘要： 目的 探讨甾体皂苷TSA对脑缺血再灌注大鼠恢复早期运动感觉功能的影响。方法 建立大鼠大脑中动脉缺血再灌注模型，随机分为模型组，TSA 15 mg/kg、30 mg/kg、60 mg/kg给药组，安宫牛黄丸400 mg/kg组以及假手术组。于造模后3～14 d给药，术后3 d、7 d、10 d、14 d观察横木行走功能和揭除胶布的潜伏期，观察皮层运动感觉区神经细胞的形态结构，免疫组化法检测脑梗死周围区血管内皮生长因子（VEGF）的表达以及微血管密度。结果 与模型组比较，TAS 30 mg/kg、60 mg/kg给药组能够增强脑缺血大鼠横木行走的能力（P<0.05），缩短揭除胶布的潜伏期（P<0.05），减轻皮层运动感觉区的神经细胞损伤，升高梗死周围区VEGF的表达以及微血管的数目。结论 TSA可加快大鼠局灶性脑缺血后运动感觉功能的恢复，其可能的机制是促进了大脑VEGF的表达和微血管的新生。

关键词: 脑缺血, 甾体皂苷, 感觉运动功能, 血管内皮生长因子（VEGF）, 血管新生, 大鼠

Abstract: bjective To investigate the effect of Steroidal Saponin TSA on the recovery of sensorimotor function after cerebral ischemia in rats. Methods Cerebral ischemia was induced by middle cerebral artery occlusion in rats which were divided into 6 groups: vehicle, TSA 15 mg/kg, 30 mg/kg, 60 mg/kg，Angong Niuhuang (ANGH) 400 mg/kg and sham. Animals received drug administration 3～14 d after ischemia. Sensorimotor function was evaluated with beam-walking and adhesivetape-exposing performance 3 d, 7 d, 10 d, and 14 d after ischemia. Neural cell injury in the sensorimotor cortex ipsilateral to ischemia was studied with hematoxylin-eosin stain. Immunohistochemistry Methods were used to measure the vascular endothelial growth factor (VEGF) protein and the angiogenesis of the area around infarction tissue. Results Animals receiving TSA at a dosage of 30 mg/kg and 60 mg/kg recovered better in beam-walking and adhesivetape-exposing performance than vehicles, and the improvement became significant 14 d after ischemia. Treatment with TSA 30 mg/kg、60 mg/kg also significantly reduced the neural cell loss in sensorimotor cortex and increased the expression of VEGF protein and the microvascular density. Conclusion TSA can improve the recovery of sensorimotor function follwing focal cerebral ischemia in rats, which may involve the neural cells protection, promoting the expression of VEGF and angiogenesis after ischemia.

Key words: cerebral ischemia, steroidal saponin, sensorimotor function, vascular endothelial growth factor (VEGF), angiogenesis, rats