《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (3): 240-242.

• 论文 • 上一篇    下一篇

脂多糖致新生鼠脑白质损伤后Bcl-2和P53蛋白表达的变化

马庆春1，李林2   

  1. 1.佳木斯大学康复医学院,黑龙江佳木斯市 154007；2.广州康复实验学校,广东广州市 510630。
  • 收稿日期:2010-11-01 修回日期:2010-12-23 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 李林

Expression of Bcl-2 and P53 in Neonate Rats with White Matter Damage Induced by Lipopolysaccharide

MA Qing-chun, LI Lin.   

  1. Rehabilitation Medicine College of Jiamusi University, Jiamusi 154007, Heilongjiang, China
  • Received:2010-11-01 Revised:2010-12-23 Published:2011-03-25 Online:2011-03-25

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摘要： 目的探讨脂多糖致新生鼠脑白质损伤(PVL)后Bcl-2、P53蛋白的变化。方法新生2日龄Wistar大鼠100只，随机分为2组：对照组和PVL组，每组50只。对照组给予生理盐水5 mg/kg腹腔注射，PVL组给予脂多糖5 mg/kg腹腔注射。免疫组化染色观察术后6 h、12 h、24 h、48 h Bcl-2及P53蛋白的变化。结果PVL组Bcl-2蛋白表达6 h升高，12 h明显升高，48 h达高峰，与对照组同一时间点比较均有非常显著性差异（P<0.01）；P53蛋白表达6 h明显升高，12 h达高峰，与对照组同一时间点比较均有非常显著性差异（P<0.01）。结论脂多糖致早期新生鼠脑白质损伤增加Bcl-2和P53的表达。

关键词: 脑白质损伤, Bcl-2, P53, 大鼠

Abstract: ObjectiveTo investigate the change of Bcl-2 and P53 expresssion after whiter matter damage in neonatal rats. Methods100 rats (2 days old) were randomly divided into two groups: control group and periventricular leucumalacia (PVL) group. Control group received normal saline 5 mg/kg intraperitoneal injection, PVL group received lipopolysaccharide (LPS) 5 mg/kg intraperitoneal injection. The expression of Bcl-2 and P53 was measured 6 h, 12 h, 24 h, 48 h after operation with immunohistochemical staining. ResultsThe expression of Bcl-2 increased 6 h after operation, and to the top 48 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). The expression of P53 increased at 6 h after operation, and to the top 12 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). ConclusionThe expression of Bcl-2 and P53 increased in the white matter injuried neonatal rats induced by LPS.

Key words: whiter matter damage, Bcl-2, P53, rats