《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (4): 324-328.

• 基础研究 • 上一篇    下一篇

经颅和经脊髓重复磁刺激对脊髓半横断大鼠运动功能的作用

潘钰1，汪璇2，刘萍2，陈赞3a，王玉兰3b   

  • 收稿日期:2012-10-30 修回日期:1900-01-01 出版日期:2013-04-25 发布日期:2013-04-25

Effects of Repetitive Transcranial Magnetic Stimulation and Magnetic Stimulation over Spinal Cord on Motor Function Recovery after Spinal Cord Hemisection in Rats

PAN Yu, WANG Xuan, LIU Ping, et al.   

  1. Department of Rehabilitation, Beijing Rehabilitation Center,Beijing, 100144, China
  • Received:2012-10-30 Revised:1900-01-01 Published:2013-04-25 Online:2013-04-25

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被引次数

10

摘要： 目的　观察经颅和经脊髓重复磁刺激对脊髓半横断大鼠运动功能的影响。方法　建立T10大鼠脊髓半横断模型，脊髓半横断大鼠于术后4 d 分别给予经颅和经脊髓重复磁刺激治疗，刺激强度为最大输出强度的35%，刺激频率5Hz，每序列5s，间歇2min，连续10个序列，每天1 次，每周5 d，连续2周。各组大鼠治疗前和治疗后进行BBB评分和水平梯子实验评价运动功能；术后38d取患侧下肢胫前肌行降钙素基因相关肽免疫组织化学染色。结果　经颅磁刺激组大鼠手术17d后各时间点BBB评分和水平梯子实验步态正确率高于术后3d和脊髓损伤组(P<0.05)；经脊髓磁刺激组大鼠手术10 d 后各时间点BBB评分和步态正确率高于术后3 d和脊髓损伤组(P<0.05)，术后10d步态正确率高于经颅磁刺激组(P<0.05)。经颅和经脊髓磁刺激组大鼠胫前肌运动终板降钙素基因相关肽表达显著高于脊髓损伤组(P<0.001)。结论　早期经颅和经脊髓磁刺激可促进脊髓半横断大鼠运动功能改善，并改变远端肌肉的可塑性，经脊髓磁刺激可加速脊髓半横断大鼠运动功能恢复。

关键词: 经颅磁刺激, 经脊髓磁刺激, 脊髓半横断, 运动功能, 恢复, 大鼠

Abstract: Objective To observe the effect of repetitive transcranial magnetic stimulation (rTMS) and functional magnetic stimulation over spinal cord (SC-MS) on motor function recovery after spinal cord hemisection in rats. Methods T10 spinal cord hemisection model was made. The stimulation (5×10 s bursts of 5 Hz at 35% maximal stimulator output, each burst separated by a 2 m interval) was delivered daily, 5 d per week for 2 weeks. The treatment began at 4 d after surgery for rTMS group and SC-MS group. Motor function recovery was assessed with Basso, Beattie & Bresnahan locomotor rating scale (BBB) and the Horizontal Ladder test. The tibialis anterior was surgically removed at 38 d after spinal cord injury for calcitonin gene-related peptide (CGRP) iummunohistochemical staining. Results The scores of BBB and Horizontal Ladder test were significantly more at 17 d after spinal cord hemisection in rTMS group than before treatment and in spinal cord injury group (P<0.05). In SC-MS group, the scores of BBB and Horizontal Ladder Test were significantly more 10 d after SCI than before treatment and in SCI group (P<0.05). The score of Horizontal Ladder test of SC-MS group was more 10 d after SCI than that in the rTMS gourp (P<0.05). The expression of CGRP on motor endplates of the tibialis anterior in rTMS group and SC-MS group were more than those of SCI group (P<0.01). Conclusion rTMS and SC-MS in acute stage can improve the motor function recovery and muscle plasticity after spinal cord hemisection in rats. The magnetic stimulation can facilitate the recovery of motor function after spinal cord hemisection in rats.

Key words: repetitive transcranial magnetic stimulation, magnetic stimulation over spinal cord, spinal cord hemisection, motor function, recovery, rats