《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 397-401.

• 基础研究 • 上一篇    下一篇

银杏叶提取物对脊髓损伤后大鼠肠道功能的影响

李欣芮，罗兰，杨拯，王强，宋强，陈勇，黄倩，聂欢，陈建敏
   

  1. 成都医学院基础医学实验教学中心，四川成都市 610500。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Effect of Ginkgo Biloba Extract on Intestinal Function after Spinal Cord Injury in Rats

LI Xin-rui, LUO Lan, YANG Zheng, WANG Qiang, SONG Qiang, CHEN Yong, HUANG Qian, NIE Huan, CHEN Jian-min
   

  1. Chengdu Medical College, Chengdu, Sichuan 610500, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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摘要： 目的 观察银杏叶提取物舒血宁对脊髓损伤后大鼠肠道功能变化的影响。方法 36只 Sprague-Dawley大鼠，运用随机数字表法随机分为A组、B组和C组，每组12只。采取Allen法(10 g×25 mm)损伤大鼠T10脊髓节段。造模成功30 min后，A组腹腔注射甲基强的松龙 30 mg/kg，此后每天 1次；B组腹腔注射舒血宁 1.75 mg/kg，此后每天 1次；C组腹腔注射等体积生理盐水。术后1 d、3 d、7 d随机抽取各组动物4只，进行大鼠肠肌电慢波活动测试，测定血清中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量，HE染色观察肠组织形态。结果 术后3 d、7 d，A、B组肌电慢波振幅和频率值均高于C组(P<0.05)，血清内SOD活性均高于C组(P<0.05)，MDA含量均明显低于C组(P<0.01)。A、B组回肠绒毛损伤较C组轻(P<0.05)，炎症渗出不显著，出血点少且出血面积局限，C组绒毛变钝或有裂隙，伴有大量炎性细胞浸润，黏膜表面大量炎性渗出物。结论 舒血宁通过其抗氧化功能，促进损伤脊髓后的肠道功能障碍恢复。

关键词: 银杏叶提取物, 脊髓损伤, 丙二醛, 超氧化物歧化酶, 肠道功能障碍

Abstract: Objective To observe the effect of Ginkgo biloba extract (EGb) on intestinal function after spinal cord injury (SCI) in rats. Methods 36 Sprague-Dawley rats were randomly divided into group A (n=12), group B (n=12) and group C (n=12). SCI model was established with Allen's mode (10 g×25 mm) at T10. 30 minutes later, group A was intraperitoneally injected with methylprednisolone 30 mg/kg every 24 hours; group B was injected with Shuxuening injection (EGb) 1.75 mg/kg every 24 hours; group C were injected with equal volume of saline. The slow wave of intestinal smooth muscle was measured, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum were determined 1 day, 3 days and 7 days after modeling, while intestinal tissue was tested with HE staining. Results The amplitude and frequency of the myoelectric slow wave increased in the groups A and B 3 and 7 days after modeling compared with those in the group C (P<0.05); meanwhile, the activity of SOD increased and content of MDA decreased in the groups A and B (P<0.05). The HE scores decreased in the groups A and B compared with those in the group C (P<0.05), which presented that the inflammatory exudation was mild, the hemorrhagic spot was few and the area was limited. The intestinal villous of the group C was blunt with large infiltration of inflammatory cells and inflammatory exudate on the mucosal surface. Conclusion EGb can improve the recovery of intestinal function in rats spinal cord injury through antioxidant.

Key words: Ginkgo biloba extract, spinal cord injury, malondialdehyde, superoxide dismutase, gastrointestinal dysfunction